Wyniki wyszukiwania

1

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Effects of antibiotics on acquired immunity in vivo - current state of knowledge

100%

Pomorska-Mol M., Pejsak Z.

Polish Journal of Veterinary Sciences

|

2012

|

tom 15

|

nr 3

Antibiotics are widely used in the therapy of infections. Besides the respective interactions between antibiotics and pathogens it seems that antibiotics also directly interact with the immune system. Some commonly used antibiotics are currently known to have effects on the innate immune response, as shown by in vitro, ex vivo and also in vivo animal experiments and clinical studies. Most of the experimental papers published to date, as well as most reviews, relate to how antibiotics affect the innate immune response or non-specific monocyte or lymphocyte proliferation. However the effects of antibiotics on the adaptive immune response are still not well characterized. This review of the literature considering different in vivo experiments indicate the real importance of interrelations existing between acquired immune responses and antibiotics, however, the mechanism of immunomodulatory effects of antibiotics are still poorly understood. Currently, data on the immunomodulating effects of antibiotics often remain heterogeneous, contradictory or insufficient, but most results published to date revealed the immunosuppressive effect of antibiotics on the antigen- specific immune response in vivo. In pigs as well as in poultry herds, it is not uncommon practice to add antibiotics to drinking water or feed at the time of vaccination. Information on the effects of such practices on the immune system of animals is restricted and more in vivo studies are needed to investigate the effects of antimicrobial drugs on the immune system, especially in the field conditions.

2

The knockdown of c-myc expression by RNAi inhibits cell proliferation in human colon cancer HT-29 cells in vitro and in vivo

72%

Zhang X., Ge Y.-L., Tian R.-H.

Cellular and Molecular Biology Letters

|

2009

|

tom 14

|

nr 2

305-318

We investigated the effects of RNA interference-mediated silencing of the c-myc gene on celluar proliferation and apoptosis in human colon cancer HT-29 cells in vitro and in vivo. A small interfering RNA (siRNA) targeting c-myc was designed, the DNA template was synthesized, and the siRNA was obtained by in vitro transcription. After siRNA transfection into HT-29 and human neuroblastoma IMR-32 cells with Lipofectamine 2000™, the proliferation of the HT-29 and IMR-32 cells was assessed via 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) colorimetry, and Hoechst 33258 staining was used to observe cell apoptosis. Following gene transfer to HT-29 cells, the expression of c-myc mRNA was examined via reverse transcription polymerase chain reaction, and the level of the protein via Western blot assay. Growth curves were constructed and in vivo experiments were performed on nude mice to assess the effects of c-myc silencing on tumor growth. The c-myc expression in the tumor tissue was measured by reverse transcription polymerase chain reaction and subsequently by immunohistochemistry. Our paper demonstrates that the delivery of siRNA directed against c-myc not only efficiently down-regulated the expression of c-myc, inhibited the proliferation of HT-29 cells and induced apoptosis in vitro, but also suppressed the growth of colon cancer cells in vivo.

3

Effect of aluminium and copper on the development of birch (Betula pendula Roth.) cultured in vitro and in vivo

58%

Bojarczuk K., Szczygiel K.

Dendrobiology

|

2004

|

tom 51

3-8

Adventitious bud cultures were established by using buds of a selected birch clone (Betula pendula Roth.) resistant to industrial pollution. The Murashige and Skoog medium (1/2 and 1/4 MS) was used for multiplication and rooting of shoots. Aluminium was added to the medium, in the form of aluminium sulphate (50–100 mg Al dm–3), and birch culture was continued in vitro for over 12 months. The shoots developed on media with aluminium (Al+) proved to be more tolerant to aluminium and copper (added to the medium as nitrates or sulphates, at a concentration of 0.05–2.0 mM) during multiplication and rooting than those developed on media without aluminium (Al–). Rooted birch microcuttings obtained from cultures on media with aluminium (Al+) grew better in the soil from an unpolluted area (Zwierzyniec, Z) and from an area polluted by a phosphate fertilise factory (Luboń, L) than those from media without aluminium (Al–).

4

Influence of selected antibiotics on interferon synthesis

58%

Zielinska-Jenczylik J., Sypula A.

Archivum Immunologiae et Therapiae Experimentalis

|

1989

|

tom 37

|

nr 1-2

5

Sklad kwasow tluszczowych w lisciach i korzeniach mieszancow Brassica oleraceae ssp. x Brassica napus oraz rzepaku rosnacych w warunkach in vitro oraz in vivo

58%

Starzycki M., Starzycka E., Czernik-Kolodziej K.

Rośliny Oleiste - Oilseed Crops

|

2000

|

tom 21

|

nr 2

625-630

6

Impact of contrasting fish oil concentrations in the diet on ovine embryo development in vivo and of corresponding diet-specific derivative sera during in vitro culture

58%

McEvoy T. G., Onal A. G., Speake B. K., Robinson J. J.

Journal of Animal and Feed Sciences

|

2012

|

tom 21

|

nr 1

In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6% w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F)% fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32% of blastocysts) than with C (27%; ns) or 6F (16%; P<0.05) or FCS (10%; P<0.005). It is suggested that at less than 3% inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.

Ograniczanie wyników

1 Archivum Immunologiae et Therapiae Experimentalis

1 Cellular and Molecular Biology Letters

1 Dendrobiology

1 Journal of Animal and Feed Sciences

1 Polish Journal of Veterinary Sciences

1 Rośliny Oleiste - Oilseed Crops

1 Bojarczuk K.

1 Czernik-Kolodziej K.

1 Ge Y.-L.

1 McEvoy T. G.

1 Onal A. G.

1 Pejsak Z.

1 Pomorska-Mol M.

1 Robinson J. J.

1 Speake B. K.

1 Starzycka E.

1 Starzycki M.

1 Sypula A.

1 Szczygiel K.

1 Tian R.-H.

1 Zhang X.

1 Zielinska-Jenczylik J.

2 2012

1 2009

1 2004