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The absolute and relative levels of immunoglobulins (Ig) G, M, and A in porcine mammary secretions throughout lactation period were estimated. Concentrations of each immunoglobulin were determined by ELISA kits. The results of the study confirmed that pigs, like other mammals, have colostral and milk phases of lactation, distinguished by the concentrations of IgG and IgA. The amount of IgG showed a dramatic 27-fold decline from the beginning to day 6 of lactation. The IgM concentration decreased over threefold at the same time. Concentration of IgA at the day 6 of lactation was almost 4-fold lower than that at the beginning of lactation. The relative contribution of IgG and IgA to the total Ig content becomes reversed during lactation. Summarising, in pigs, transformation of colostrum to milk was characterised by dramatic decrease in total Ig content, connected with the change of predominant immunoglobulin. The predominant immunoglobulin in colostrum was IgG, which plays an important role in systemic immunity, while in milk a major Ig was IgA, responsible for the protection at mucosal surface level. As concentrations of Ig in colostrum are very variable, an improved understanding of factors influencing colostrum Ig concentration is now desirable.
The aim of this study was to determine the titre of IgG₁, IgG₂, and lgM antibodies against bovine leukaemia virus (BLV) in the serum of either experimentally or naturally infected animals. Experimental infection performed in calves injected intravenously with blood from leukaemic cattle. The presence of BLV antibodies belonging to lgG₁, IgG₂, and IgM classes were studied for 7 months using the ELISA. Subsequent examinations were carried out on 126 cows from breeding stations extensively affected with BLV. Serological examinations of antibodies against BLV was performed by the ELISA. In the sera of experimentally infected animals IgM antibodies attained a maximum level in stage 2 (42 to 64 d) but lgG₁ antibodies during the last stage of immunogenesis ( 182 to 224 d). No statistically significant differences in IgG₂ antibodies throughout the entire experimental period were found. In the case of natural infection the IgG₁ and IgG₂ antibody levels increased concomitantly with the total antibody titres in the serum; however, IgG₂ values were significantly lower throughout the entire period of the study. In contrast, the highest lgM titre value was found in animals with the lowest level of total antibodies. Enzootic bovine leukaemia (EBL) is a neoplastic disease, associated with unlimited and irreparable proliferous alterations induced by bovine leukaemia virus (BL V) (25). Lymphocytes B are regarded as the target cells although T lymphocytes may also be infected (27, 30). Bovine leukosis reveals a long-lasting latency period involving integration of the viral genetic material with the host cell genome in the proviral form. A chronic lymphocytosis develops in about 30% of infected animals (1, 6, 27) whereas a small percentage of infected cattle develops tumours (6, 7). The production of antibodies against BLV is a permanent feature in the affected animals. The influence of BLV infection on immunoglobulin levels in sera has been extensively investigated but the results are contradictory (8, 9, 12, 13, 22, 29, 31). These investigations have mainly focused on total lg level measurements; consequently conflicting results concerning the behaviour of immunoglobulins regarded as the specific antibodies to the virus have been obtained.
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