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1

Antigen recognition patterns of intestinal capillariasis using immunoblot-based serodiagnosis

100%
Hassan M. A., Basyoni M. M. A., Amer M. F., Abdel- Ghany Al-Antably A. S.
Acta Parasitologica
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2020
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tom 65
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nr 4
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Evaluation of human cystic echinococcosis before and after surgery and chemotherapy by demonstration of antibodies in serum

84%
Tenguria R. K., Naik M. I.
Annals of Parasitology
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2014
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tom 60
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nr 4
Human cystic echinococcosis (CE), caused by Echinococcus granulosus, is one of the most important and widespread parasitic zoonoses. As one of the problems that can be encountered after treating CE patients is the risk of postsurgical relapses or treatment failure, a long-term clinical and serological follow-up is required to evaluate the success and failure of therapy. Therefore, the aim of the present study was to identify the best diagnostic and prognostic ELISA markers in patients with CE. The cohort comprised 50 patients with symptomatic CE treated with antihelminthic drugs and surgery, who were followed up clinically and radiologically for a mean of 6 years (range 4–8 years). The results clearly indicate that the hydatid specific antibodies of IgE, IgG1 and IgG4 are the most important antibodies for the serological diagnosis of cystic echinococcosis during the active stage of the disease. None of the serum samples from healthy controls gave a non-specific reaction with IgE, IgG1 or IgG4, and a considerably reduced cross-reaction was observed with these antibodies. During post-operative follow-up, the IgM, IgE, IgG1, IgG2 and IgG4 antibody response provided the best correlate of disease activity. The detection of total IgG and IgG3 subclass antibody response for the assessment of post-treatment disease activity among CE patients was insensitive. All patients responded to treatment except 2 women (32 and 36 years old), in whom multiple cysts (12 and 7 cysts) were detected in the liver and lung two years after the first operation. Hence, it can be concluded that the CE-specific antibodies of IgE, IgG1 and IgG4 are the best immunological markers for diagnosis and prognosis of CE patients.
3
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Survey of equine cryptosporidiosis in Poland and the possibility of zoonotic transmission

84%
Majewska A. C., Werner A., Sulima P., Luty T.
Annals of Agricultural and Environmental Medicine
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1999
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tom 06
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nr 2
4

Prevalence of avian Chlamydophila psittaci in China

84%
Yang J., Yang Q., Yang J., He C.
Bulletin of the Veterinary Institute in Puławy
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2007
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tom 51
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nr 3
Examinations were carried out in 46 intensive farms in northern China to investigate avian Chlamydophila psittaci. Five hundred and twenty-five avian sera were selected for examining antibodies to C. psittaci by ELISA. One hundred and fifty-five clinical samples from throat swabs and oviduct tissues were tested for the presence of chlamydial antigen using IDEIA™ PCE chlamydia dual amplification immunoassay, and 60 samples were tested by ompA gene-based PCR. C. psittaci antibodies were detected in 387 (77.8%) out of 525 serum samples, with seroprevalences ranging from 50% to 100%. Among the tested samples, 98/150 (65.3%) in broilers, 173/210 (82.3%) in ducks, and 116/165 (70.3%) in laying hens were detected to be positive, respectively. Using PCE-ELISA test kits, in 91 out of 155 clinical samples the presence of antigen was confirmed, while 64 samples were negative. Forty-three PCR's were tested as positive out of 60 samples, while 17 samples were confirmed to be negative. Both higher positive antibodies and the presence of antigens were found in avian flocks associated with typical clinical signs suggestive of chlamydiosis. This study showed a severe prevalence of C. psittaci among different species of domestic birds in China.
5

Role of Toxoplasma gondii in abortion od ewes in Ahvaz [South-West Iran]

84%
Hamidinejat H., Goraninejad S., Ghorbanpoor M., Nabavi L., Akbarnejad F.
Bulletin of the Veterinary Institute in Puławy
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2008
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tom 52
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nr 3
To investigate the role of Toxoplasma gondii in abortion of ewes in Ahvaz, South-West Iran, a commercial ELISA kit and a modified agglutination test (MAT) were used for the detection of antibodies to Toxoplasma gondii in ewes. Out of 150 examined ewes, 100 of them had recently aborted and 50 did not have any abortion history. Toxoplasma gondii antibodies were detected in 109 (72.6%) ewes by ELISA and in 104 (69.3%) ewes by MAT. Seropositivity in the group with abortion history was 85% by ELISA and 80% by MAT and in the group with no abortion history was 58%, by both ELISA and MAT. Good correlation was seen between the ELISA and MAT results. The results suggested that the ELISA and MAT were good tools for epidemiological studies of Toxoplasma gondii infection in sheep. Toxoplasma gondii may play an important role in ewe's abortion in Ahvaz.
6

Monoclonal antibodies - selected problems

84%
Furowicz A. J., Karakulska J., Silecka A.
Advances in Agricultural Sciences
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2002
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tom 08
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nr 1
7

Clinical, epidemiological and laboratory criteria for the diagnosis of human cysticercosis in Brazilian patients

84%
Cavalcante M. M. F. P., Cavalcanti J. E., Costa-Cruz J. M., Barbosa A. P., Silva S. A., Campos D. M. B.
Acta Parasitologica
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2006
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tom 51
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nr 1
Cysticercosis (CC) is a polymorphous disease, which makes its diagnosis difficult. This study had the objective of evaluating the clinical, epidemiological and laboratory criteria in human CC. An epidemiological questionnaire was applied, and indirect fluorescence antibody test (IFAT) and ELISA-IgG were utilized together with computerized tomography and/or magnetic resonance imaging on 90 patients with clinical signs suggestive of neurocysticercosis (NCC). Most patients had previously lived under deficient basic sanitary conditions. The imaging techniques showed that 92.2% of the cysticerci were in the cerebral parenchyma, 5.5% had a ventricular location, 1.1% were periventricular and 1% was ocular. The cysticerci were observed to be predominantly in the inactive phase. Seropositivity to the IFAT and/or ELISA was shown by 32.2% (29/90). Of the 29 seropositive patients, 72.4% presented cysticerci in the inactive form, and of the 61 seronegative patients, 78.7% also presented cysticerci in the inactive form. There was no correlation between active CC and seropositivity, since 72.4% of the seropositive patients presented calcified cysticerci. The results demonstrated that imaging techniques contributed significantly to elucidate the laboratorial diagnosis and to evaluate the stage of cysticercus development.
8

Sequential histopathological changes and cytokine expressions in dogs naturally infested with Sarcoptes scabiei mites

67%
Nwufoh O. C., Sadiq N. A., Adediran O. A., Jarikre T. A., Emikpe B. O.
Acta Parasitologica
|
2020
|
tom 65
|
nr 2
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