Wyniki wyszukiwania

1

Comparison of the trabeculae structure of the spongy bone of the bilateral pastern bones in racehorses based on the imaging analysis of radiograms

100%

Dzierzecka M., Czerwinski E.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 3

2

Morphology of the pulmonary valve [Valva trunci pulmonali] in chosen species of domestic and wild birds using imaging method

100%

Bartyzel B. J.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

303-308

The morphological types of the pulmonary valve and its adjacent structures were determined. The research was conducted on the heart of 498 domestic and wild birds of five orders. The organ underwent imaging with the use of an X-ray apparatus, a 16- slice computed tomography scanner, and an ultrasonograph. The structures examined are characterised by a great changeability as far as particular taxonomie individuals are concerned. This is connected with the ways and living conditions of particular taxonomic and ecological groups of birds. New terms to characterise the pulmonary valve were suggested. Research of this type will allow us to link such basic sorts of study with cardiac surgery in the future.

3

Modulation of CCK-8-evoked intracellular Ca2+ waves by hydrogen peroxide in mouse pancreatic acinar cells

100%

Granados M. P., Salido G. M., Pariente J. A., Gonzalez A.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 3

In the present study we have employed single cell imaging analysis to monitor the propagation of cholecystokinin-evoked Ca2+ waves in mouse pancreatic acinar cells. Stimulation of cells with 1 nM CCK-8 led to an initial Ca2+ release at the luminal cell pole and subsequent spreading of the Ca2+ signal towards the basolateral membrane in the form of a Ca2+ wave. Inhibition of sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) activity by 1 µM thapsigargin, preincubation in the presence of 100 µM H2O2 or inhibition of PKC with either 5 µM Ro31-8220 or 3 µM GF-109203-X all led to a faster propagation of CCK-8-induced Ca2+ signals. The propagation of CCK-8-evoked Ca2+ signals was slowed down by activation of PKC with 1 µM PMA, and preincubation of cells in the presence of H2O2 counteracted the effect of PKC inhibition. The protonophore FCCP (100 nM) and the inhibitor of the mitochondrial Ca2+-uniporter Ru360 (10 µM) led to an increase in the propagation rate of CCK-8-evoked Ca2+ waves. Finally, depolymerisation of actin cytoskeleton with cytochalasin D (10 µM) led to a faster propagation of CCK-8-evoked Ca2+ signals. Stabilization of actin cytoskeleton with jasplakinolide (10 µM) did not induce significant changes on CCK-8-evoked Ca2+ waves. Preincubation of cells in the presence of H2O2 counteracted the effect of cytochalasin D on CCK-8-evoked Ca2+ wave propagation. Our results suggest that spreading of cytosolic Ca2+ waves evoked by CCK-8 can be modulated by low levels of oxidants acting on multiple Ca2+-handling mechanisms.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Journal of Physiology and Pharmacology

1 Polish Journal of Veterinary Sciences

1 Bartyzel B. J.

1 Czerwinski E.

1 Dzierzecka M.

1 Gonzalez A.

1 Granados M. P.

1 Pariente J. A.

1 Salido G. M.

1 2010

1 2009

1 2007

Comparison of the trabeculae structure of the spongy bone of the bilateral pastern bones in racehorses based on the imaging analysis of radiograms

Morphology of the pulmonary valve [Valva trunci pulmonali] in chosen species of domestic and wild birds using imaging method

Modulation of CCK-8-evoked intracellular Ca2+ waves by hydrogen peroxide in mouse pancreatic acinar cells