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Polyphenol composition of extract from aerial parts of Circaea lutetiana L. and its antioxidant and anti-inflammatory activity in vitro

100%

Granica S., Piwowarski J. P., Kiss A. K.

Acta Biologica Cracoviensia. Series Botanica

2013

tom 55

nr 1

The chemical composition and bioactivity of a water/methanol extract prepared from aerial parts of Circaea lutetiana were determined. HPLC-DAD-MS3 analysis revealed the presence of 14 different compounds comprising phenolic acids, ellagitannins and flavonoids. Antioxidant assays showed the extract's strong scavenging activity towards DPPH (SC50 33.1±3.1 μg/ml), O2 - (SC50 4.0±2.3 μg/ml) and H2O2 (SC50 below 2 μg/ml). Enzyme-based studies revealed that Circaea lutetiana extract inhibits the activity of hyaluronidase (IC50 13.3±2.4 μg/ml) and lipoxygenase (IC50 44.7±1.4 μg/ml). These results support some traditional uses of Circaea lutetiana.

Hyaluronidase in human somatic tissues and urine: polymorphism and the activity in diseases

100%

Fiszer-Szafarz B., Vannier P., Litynska A., Zou L., Czartoryska B., Tylki-Szymanska A.

Acta Biochimica Polonica

1995

tom 42

nr 1

The polymorphism of hyaluronidase (EC 3.2.1.35) (Hyase) was studied on a hyalu- ronan-polyacrylamide gel. Liver, placenta, ovary and breast tissue were found to have 7 active isoforms while leukocytes and platelets 5 and fibroblasts displayed no hyaluronidase activity. In serum, synovial fluid and urine soluble the most acidic forms are present. Desialylation showed that most of the hyaluronidase isoforms differ in the content of sialic acid. In patients with rheumatoid arthritis, hyaluronidase activity in the synovial fluid varied from not detectable to very high. A partial deficiency was demnostrated in sera from some patients with dysostosis multiplex without mucopolysacchariduria. In I-cell disease, hyaluronidase activity in serum was as that in controls.

Age changes of fructose and citric acid levels as well as hyaluronidase activity in the epididymal secretion and vesicular glands of the Podolsky plant type bulls of the Ukrainian black-dappled dairy breed

67%

Pavlyuk N., Vovk S.

Acta Scientiarum Polonorum. Zootechnica

2013

tom 12

nr 3

This study presents data on the age changes of hyaluronidase activity in epididymal secretion as well as the presence of fructose and citric acid in vesicular glands (glandulae vesiculares) secretion. The results of age changes in the amount of epididymal secretion and its spermatozoa concentration in the Podolsky plant type bulls of the Ukrainian black-dappled dairy breed are also given. The research was conducted on 54 individuals, aged 1--13 months. It was found that fructose and citric acid in vesicular glands secretion as well as hyaluronidase activity and mature spermatozoa in epididymal secretion emerge for the first time in 5-month-old bulls. The highest concentrations of fructose (742 ±63 mg/per cent) and citric acid (914 ±81 mg/per cent) in vesicular glands secretion and the highest hyaluronidase activity (1.6 thousand units) in epididymal secretion were found in 12-month-old bulls. A strong positive correlation between hyaluronidase activity and spermatozoa concentration in epididymal secretion was also observed.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Scientiarum Polonorum. Zootechnica

1 Czartoryska B.

1 Fiszer-Szafarz B.

1 Granica S.

1 Kiss A. K.

1 Litynska A.

1 Pavlyuk N.

1 Piwowarski J. P.

1 Tylki-Szymanska A.

1 Vannier P.

1 Vovk S.

1 Zou L.

2 2013

1 1995

Wyniki wyszukiwania

Polyphenol composition of extract from aerial parts of Circaea lutetiana L. and its antioxidant and anti-inflammatory activity in vitro

Hyaluronidase in human somatic tissues and urine: polymorphism and the activity in diseases

Age changes of fructose and citric acid levels as well as hyaluronidase activity in the epididymal secretion and vesicular glands of the Podolsky plant type bulls of the Ukrainian black-dappled dairy breed