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1

The effect of different nucleoside-5'-thiosulphates on the salvage of deoxypyrimidine nucleosides in human lymphocytes

100%

Szikla K., Kazimierczuk Z., Virga S., Staub M.

Cellular and Molecular Biology Letters

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1999

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tom 04

|

nr 3

2

Effect of Pseudomonas aeruginosa exotoxin a on PHA-induced lymphocyte proliferation

86%

Michalkiewicz J., Patzer J., Dzierzanowska D., Stachowski J., Madalinski K.

Archivum Immunologiae et Therapiae Experimentalis

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1989

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tom 37

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nr 3-4

3

Estiamtion of BrdUrd-independent sister chromatid exchanges and proliferation rate of human lymphocytes in vitro

86%

Kotecki M., Pawlak A. L.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1989

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tom 37

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nr 10-12

4

Evaluation of the immunomodulatory activity of four compounds exerting antimutagenic effects on human lymphocytes in vitro

86%

Gasiorowski K., Brokus B., Tabaka H.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 4

469-481

Four compounds previously described as antimutagenic for human lymphocytes in vitro were tested on their immunomodulatory activity in lymphocyte cultures. The standard immunocytochemical methods were applied for microscopic examination of the percentual representation of the main lymphocyte subpopulation. The results imply that all of the tested compounds exhibited significant immunomodulatory effect, with that of fluphenazine being the strongest, whereas that of todralazine is the weakest. Two of the tested compounds: anthocyanins from Aronia melanocarpa fruit, and alkylresorcinols from cereal grains, also exhibited a distinct immunomodulatory activity, and it deserves adequate attention as an activity exerted by natural products, commonly present in regular human diet. The analysis of the proliferating cell fraction, and the estimation of the cell proliferation rate suggest that the effect of the tested compounds might depend on an increase in the number of lymphocytes which expressed their differentiation antigens on the cell membranes.

5

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Influence of cysteine on mechlorethamine - induced chromosomal aberrations in cultured human lymphocytes

86%

Blaszczyk A.

Journal of Applied Genetics

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1995

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tom 36

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nr 4

The aim of the present paper was to find out by in vitro chromosomal aberration test using human lymphocytes whether cysteine has anticlastogenic properties towards a well-known mutagen - mechlorethamine. The lymphocytes tested were obtained from three healthy donors. Two doses of cysteine (1.0 and 2.0 μg/ml) and three doses of mechlorethamine (0.1,0.2 and 0.3 μg m⁻¹) were tested. It was found that cysteine had anticlastogenic properties and that it reduced the number of metaphases with chromosomal aberrations induced by mechlorethamine.

6

UV-photoproducts induced in kappa Ig MAR increase its affinity to nuclear matrices in vitro

86%

Widlak P., Rzeszowska-Wolny J.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

7

Relationship between DNA replication and DNA repair in human lymphocytes proliferating in vitro in the presence and in absence of mutagen

86%

Szyfter K., Wiktorowicz K., Wielgosz M. S., Zajaczek S., Kujawski M., Jaloszynski P., Czub M., Markowska J.

Journal of Applied Genetics

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1995

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tom 36

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nr 4

The effects of mutagens on DNA replication and DNA repair were studied in peripheral blood lymphocytes (PBL) obtained from 21 healthy subjects, 2 samples from healthy heterozygote of Xeroderma pigmentosum (XP) and 2 samples from patient with clinically recognised XP. Inter-individual variations were found in DNA replication and in the level of spontaneous DNA repair measured under standard culture condition. Exposure of human PBL proliferating in vitro to B(a)P was followed by a partial inhibition of replicative DNA synthesis in all subjects and by an induction of DNA repair in healthy subjects. In XP patients DNA repair synthesis remained at the level attributed to spontaneous DNA repair. The response to mutagen varied individually. Results were analysed statistically. It was established that the studied indices of DNA synthesis correlate well with each other. The highest correlation was found between the levels of spontaneous and B(a)P-induced DNA repair. It is concluded that the level of spontaneous DNA repair is predictive for an estimation of cells ability to repair DNA damage. Inter-individual variations in the inhibition of DNA replication and in DNA repair synthesis are also dependent on the type of mutagen as shown by effects of other mutagens. Different effects of mutagen exposure on the inhibition of DNA replicative synthesis and induction of DNA repair can be explained by genetically controlled differences in the activity of enzymes responsible for mutagen processing and lesion removal.

8

Boric acid as a protector against paclitaxel genotoxicity

72%

Turkez H., Tatar A., Hacimuftuoglu A., Ozdemir E.

Acta Biochimica Polonica

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2010

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tom 57

|

nr 1

95-97

Paclitaxel (PAC) is an anticancer drug used for treatments of breast, ovarian and lung cancers. However, little data is; available in the literature on its potential genotoxicity on healthy human cells. On the other hand, boron deficiency and supplementation exert important biological effects in human and animal tissues. The biological effects of dietary boron are defined, but its interaction with PAC is not known for therapeutic uses. The aim of the present study was to determine whether boric acid (BA) confer a protection against PAC genotoxicity. After the application of PAC (10 or 20 μg/l) and BA (2.5 or 5 mg/l), the genotoxic effects were assessed by sister chromatid exchange (SCE) and micronucleus (MN) tests in human blood cultures. We also analyzed nuclear division index (NDI) in peripheral lymphocytes. Our results showed that PAC significantly (P < 0.05) increased the frequencies of SCEs and the formations of MNs in peripheral lymphocytes as compared to controls. PAC decreased the nuclear division index in lymphocyte cultures. Boric acid did not show cytotoxic or genotoxic effects at the concentrations tested. Furthermore, the PAC-induced increases in the genotoxicity and cytotoxicity indices were diminished by the addition of BA. The present study suggests for the first time that BA can prevent the genotoxicity of PAC on human lymphocytes.

9

Effect of insulin and glucose on adenosine metabolizing enzymes in human B lymphocytes

72%

Kocbuch K., Sakowicz-Burkiewicz M., Grden M., Szutowicz A., Pawelczyk T.

Acta Biochimica Polonica

|

2009

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tom 56

|

nr 3

439-446

In diabetes several aspects of immunity are altered, including the immunomodulatory action of adenosine. Our study was undertaken to investigate the effect of different glucose and insulin concentrations on activities of adenosine metabolizing enzymes in human B lymphocytes line SKW 6.4. The activity of adenosine deaminase in the cytosolic fraction was very low and was not affected by different glucose concentration, but in the membrane fraction of cells cultured with 25 mM glucose it was decreased by about 35% comparing to the activity in cells maintained in 5 mM glucose, irrespective of insulin concentration. The activities of 5'-nucleotidase (5'-NT) and ecto-5'-NT in SKW 6.4 cells depended on insulin concentration, but not on glucose. Cells cultured with 10-8 M insulin displayed an about 60% lower activity of cytosolic 5'-NT comparing to cells maintained at 10-11 M insulin. The activity of ecto-5'-NT was decreased by about 70% in cells cultured with 10-8 M insulin comparing to cells grown in 10-11 M insulin. Neither insulin nor glucose had an effect on adenosine kinase (AK) activity in SKW 6.4 cells or in human B cells isolated from peripheral blood. The extracellular level of adenosine and inosine during accelerated catabolism of cellular ATP depended on glucose, but not on insulin concentration. Concluding, our study demonstrates that glucose and insulin differentially affect the activities of adenosine metabolizing enzymes in human B lymphocytes, but changes in those activities do not correlate with the adenosine level in cell media during accelerated ATP catabolism, implying that nucleoside transport is the primary factor determining the extracellular level of adenosine.

10

Calcium-binding calmyrin forms stable covalent dimers in vitro, but in vivo is found in monomeric form

72%

Sobczak A., Blazejczyk M., Piszczek G., Zhao G., Kuznicki J., Wojda U.

Acta Biochimica Polonica

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2005

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tom 52

|

nr 2

The EF-hand Ca2+-binding protein calmyrin is expressed in many tissues and can interact with multiple effector proteins, probably as a sensor transferring Ca2+ signals. As oligomerization may represent one of Ca2+-signal transduction mechanisms, we characterised recombinant calmyrin forms using non-reducing SDS/PAGE, analytical ultracentrifugation and gel filtration. We also aimed at identification of biologically active calmyrin forms. Non-reducing SDS/PAGE showed that in vitro apo- and Ca2+-bound calmyrin oligomerizes forming stable intermolecular disulfide bridges. Ultracentrifugation indicated that at a 220 μM initial protein concentration apo-calmyrin existed in an equilibrium of a 21.9 kDa monomer and a 43.8 kDa dimer (trimeric or tetrameric species were not detected). The dimerization constant was calculated as Ka = 1.78 × 103 M–1 at 6oC. Gel filtration of apo- and Ca2+-bound calmyrin at a 100 μM protein concentration confirmed an equilibrium of a monomer and a covalent dimer state. Importantly, both monomer and dimer underwent significant conformational changes in response to binding of Ca2+. However, when calmyrin forms were analyzed under non-reducing conditions in cell extracts by Western blotting, only monomeric calmyrin was detected in human platelets and lymphocytes, and in rat brain. Moreover, in contrast to recombinant calmyrin, crosslinking did not preserve any dimeric species of calmyrin regardless of Ca2+ concentrations. In summary, our data indicate that although calmyrin forms stable covalent dimers in vitro, it most probably functions as a monomer in vivo.

11

Gamma-radiation-induced ATM-dependent signalling in human T-lymphocyte leukemic cells, MOLT-4

72%

Tichy A., Zaskodova D., Rezacova M., Vavrova J., Vokurkova D., Pejchal J., Vilasova Z., Cerman J., Osterreicher J.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 2

ATM kinase (ATM) is essential for activation of cell cycle check points and DNA repair in response to ionizing radiation (IR). In this work we studied the molecular mechanisms regulating DNA repair and cell death in human T-lymphocyte leukemic cells, MOLT-4. Apoptosis was evaluated by flow-cytometric detection of annexin V. Early apoptotic cells were determined as sub-G1 cells and late apoptotic cells were determined as APO2.7-positive ones. Proteins involved in ATM signalling pathway were analysed by Western-blotting. We observed a rapid (0.5 h) phosphorylation of ATM declining after 6 h after irradiation by all the doses studied (1.5, 3.0, and 7.5 Gy). Checkpoint kinase-2 (Chk-2) was also phosphorylated after 0.5 h but its phosphorylated form persisted 4, 2, and 1 h after the doses of 1.5, 3.0, and 7.5 Gy, respectively. The amount of p53 protein and its form phosphorylated on Ser-392 increased 1 h after irradiation (1-10 Gy). The lethal dose of 7.5 Gy caused an immediate induction and phosphorylation of p53 after 0.5 h post-irradiation. At the time of phosphorylation of p53, we found simultaneous phosphorylation of the oncoprotein Mdm2 on Ser-166. Neither ATM nor its downstream targets showed a dose-dependent response after 1 h when irradiated by the doses of 1-10 Gy. MOLT-4 cells were very sensitive to the effect of IR. Even low doses, such as 1.5 Gy, induced apoptosis 16 h after irradiation (evaluated according to the cleavage of nuclear lamin B to a 48-kDa fragment). IR-induced molecular signalling after exposure to all the tested doses was triggered by rapid phosphorylation of ATM and Chk-2. Subsequent induction of p53 protein and its phosphorylation was accompanied by concomitant phosphorylation of its negative regulator, oncoprotein Mdm2, and followed by induction of apoptosis.

12

The influence of protons and zinc ions on the steady-state inactivation of Kv1.3 potassium channels

72%

Teisseyre A., Mozrzymas J. W.

Cellular and Molecular Biology Letters

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2007

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tom 12

|

nr 2

Using the whole-cell patch-clamp technique, we investigated the influence of extracellular pH and zinc ions (Zn2+) on the steady-state inactivation of Kv1.3 channels expressed in human lymphocytes. The obtained data showed that lowering the extracellular pH from 7.35 to 6.8 shifted the inactivation midpoint (Vi) by 17.4 ± 1.12 mV (n = 6) towards positive membrane potentials. This shift was statistically significant (p < 0.05). Applying 100 μM Zn2+ at pH 6.8 further shifted the Vi value by 16.55 ± 1.80 mV (n = 6) towards positive membrane potentials. This shift was also statistically significant (p < 0.05). The total shift of the Vi by protons and Zn2+ was 33.95 ± 1.90 mV (n = 6), which was significantly higher (p < 0.05) than the shift caused by Zn2+ alone. The Zn2+-induced shift of the Vi at pH 6.8 was almost identical to the shift at pH = 7.35. Thus, the proton-and Zn2+-induced shifts of the Vi value were additive. The steady-state inactivation curves as a function of membrane voltage were compared with the functions of the steady-state activation. The total shift of the steady-state inactivation was almost identical to the total shift of the steady-state activation (32.01 ± 2.10 mV, n = 10). As a result, the “windows” of membrane potentials in which the channels can be active under physiological conditions were also markedly shifted towards positive membrane potentials. The values of membrane voltage and the normalised chord conductance corresponding to the points of intersection of the curves of steady-state activation and inactivation were also calculated. The possible physiological significance of the observed modulatory effects is discussed herein.

13

Ryanodine does not change biophysical properties of human lymphocyte Kv1.3 channels

72%

Teisseyre A., Mozrzymas J. W., Vais H., Usherwood P. N. R.

Cellular and Molecular Biology Letters

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1997

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tom 02

|

nr 3

Ryanodine treatment - a plant alkaloid known to be a powerful inhibitor of muscle contraction - changes the ionic selectivity and conductance of several types of K+ channels in muscle cells. Available data provide evidence that the effects of ryanodine on K+ channel properties are not secondary to Ca2+ release from sarcoplasmic reticulum, but result from a direct interaction of the alkaloid with the channel protein. In the present investigations we applied the whole-cell patch-clamp technique to study ryanodine effects on the properties of Kv1.3 channels, which are present in lymphocytes and rat brain cells. The effects of ryanodine applied in the concentration range 10-5-10-4 M on the ionic selectivity, conductance, gating and kinetics of Kv1.3 channels expressed in human T lymphocytes were examined. Our data provide evidence that none of these properties was changed upon ryanodine treatment. Altogether, our data support the notion that Ryanodine may interact with various types of K+ channels differently. The different response to Ryanodine treatment might be another pharmacological feature delineating differences among various types of K+ channels.

14

DNA-damaging effect of cadmium and protective action of quercetin

72%

Blasiak J.

Polish Journal of Environmental Studies

|

2001

|

tom 10

|

nr 6

Cadmium is a widespread environmental and occupational pollutant and quercetin is a dietary flavonoid, which is reported to modulate the effects of many mutagens and carcinogens. We investigated the ability of cadmium chloride to induce DNA damage in human lymphocytes in the presence of quercetin using the alkaline comet assay. Cadmium chloride (5-150 muM) evoked dose-dependent DNA damage and quercetin at 50 muM decreased the extent of the damage. The lymphocytes exposed to cadmium chloride were able to remove their DNA damage within a time period shorter than 120 min. The cells treated with quercetin at 50 muM prior to exposure to cadmium required shorter periods of time to recover. Quercetin could chelate cadmium ions, scavenge free radicals produced by cadmium or regenerate cellular DNA-repair enzymes.

15

Effects of intracellular cAMP on the activity of human T lymphocyte Kv1.3 channels

72%

Teisseyre A., Gawlik A., Krasnowska M.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 1

Shaker-related Kv1.3 channels are the most prevalent and widely studied ion channels in normal human T Lymphocytes (TL) as well as in certain T cell lines, such as Jurkat cells. This review focuses on modulatoty effects of intracellular cAMP on the activity of the channels. Available data provide evidence that: 1) intracellular cAMP directly activates a novel class of charybdotoxin-insensitive voltage-independent cAMP-gated K+ channels, but not the Kv1.3 channels both in quiescent and activated human T Lymphocytes, 2) intracellular cAMP reduces the Kv1.3 channel activity by protein kinase A - dependent channel phosphorylation in Jurkat TL cell line, 3) intracellular cAMP does not affect the activity of Kv1.3 channels in normal human T Lymphocytes. The apparently different effects of intracellular cAMP on Kv1.3 channels expressed in normal and Jurkat TL may reflect differences in the biochemical microenvironment as well as in an expression of auxiliary channel subunits in both cell types. A more complete biochemical characterisation of the Kv1.3 channel microenvironment and the channel-associated subunits in different T cell subtypes will be necessary to further elucidate this problem.

16

The assessment of DNA damage in lymphocytes of wooden furniture workers

72%

Palus J., Dziubaltowska E., Rydzynski K.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

Single-strand breaks (SSB) and DNA repair were detected in peripheral lymphocytes derived from workers of a furniture factory in a non-polluted region of Poland. The workers were exposed to wood dust (n = 19), or to the dust and varnishes or lacquers together (n = 5). Four groups were studied simultaneously: (a) exposed workers smokers of cigarettes (n = 14), (b) nonexposed smokers - control (n = 14), (c) exposed workers' nonsmokers (n = 14), (d) exposed nonsmokers (n = 10). In exposed workers DNA SSB and DNA repair were statistically significantly increased. DNA SSB was clearly higher in the smoking workers than in the smoking controls. Cigarette smoking itself has produced no evident increase in the frequency of DNA SSB in the control group. Occupational exposure had a significant effect on DNA repair in non stimulated lymphocytes both in smoking and nonsmoking workers.

17

Changes in membrane phospholipid biosynthesis induced by chloropromasine and nucleoside analogues: its therapeutic consequences

72%

Staub M., Sumeg R., Sasvari-Szekely M., Simmonds H. A.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

18

Changes in the enzymatic activity of glutathione S-transferases from human peripheral blood lymphocytes after the action of cis-and trans-diamminedichloroplatinum [II]

72%

Walter Z., Kargas C.

Acta Biologica Cracoviensia. Series Zoologia

|

2001

|

tom 43

19

Effects of acetylsalicylic acid and a new pyrazine derivative PD-101 on sister chromatid exchange frequency and cell kinetics in cultured human lymphocytes

72%

Wozniak A., Limon J., Petrusewicz J., Kaliszan R.

Journal of Applied Genetics

|

1998

|

tom 39

|

nr 3

Acetylsalicylic acid (ASA) and α-2-pyrazylidene-α-cyano N-butyl acetamide (PD-101), a new antiaggregatory pyrazine derivative were tested for their genotoxicity in human lymphocytes in vitro using the sister chromatid exchange (SCE) technique. Both compounds were found to be inactive in inducing SCE in concentration from 1 µM up to 1000 µM. The agents displayed inhibitory effect on cell kinetics.

20

Pyridine nucleotide concentration and activity of related enzymes in human lymphocytes

72%

Sestini S., Jacomelli G., Pescaglini M., Micheli V., Pompucci G.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

Ograniczanie wyników

The effect of different nucleoside-5'-thiosulphates on the salvage of deoxypyrimidine nucleosides in human lymphocytes

Effect of Pseudomonas aeruginosa exotoxin a on PHA-induced lymphocyte proliferation

Estiamtion of BrdUrd-independent sister chromatid exchanges and proliferation rate of human lymphocytes in vitro

Evaluation of the immunomodulatory activity of four compounds exerting antimutagenic effects on human lymphocytes in vitro

Influence of cysteine on mechlorethamine - induced chromosomal aberrations in cultured human lymphocytes

UV-photoproducts induced in kappa Ig MAR increase its affinity to nuclear matrices in vitro

Relationship between DNA replication and DNA repair in human lymphocytes proliferating in vitro in the presence and in absence of mutagen

Boric acid as a protector against paclitaxel genotoxicity

Effect of insulin and glucose on adenosine metabolizing enzymes in human B lymphocytes

Calcium-binding calmyrin forms stable covalent dimers in vitro, but in vivo is found in monomeric form

Gamma-radiation-induced ATM-dependent signalling in human T-lymphocyte leukemic cells, MOLT-4

The influence of protons and zinc ions on the steady-state inactivation of Kv1.3 potassium channels

Ryanodine does not change biophysical properties of human lymphocyte Kv1.3 channels

DNA-damaging effect of cadmium and protective action of quercetin

Effects of intracellular cAMP on the activity of human T lymphocyte Kv1.3 channels

The assessment of DNA damage in lymphocytes of wooden furniture workers

Changes in membrane phospholipid biosynthesis induced by chloropromasine and nucleoside analogues: its therapeutic consequences

Changes in the enzymatic activity of glutathione S-transferases from human peripheral blood lymphocytes after the action of cis-and trans-diamminedichloroplatinum [II]

Effects of acetylsalicylic acid and a new pyrazine derivative PD-101 on sister chromatid exchange frequency and cell kinetics in cultured human lymphocytes

Pyridine nucleotide concentration and activity of related enzymes in human lymphocytes