Wyniki wyszukiwania

1

Staphylococcus cohnii - resident of hospital environment: cell-surface features and resistance to antibiotics

100%

Szewczyk E. M., Rozalska M.

Acta Microbiologica Polonica

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2000

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tom 49

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nr 2

2

Clonal analysis of Staphylococcus aureus strains isolated in Obstetric-Gynaecological Hospital

72%

Szczuka E., Szumala-Kakol A., Siuda A., Kaznowski A.

Polish Journal of Microbiology

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2010

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tom 59

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nr 3

Epidemiological studies were carried out on 135 isolates of Staphylococcus aureus strains originating from medical staff, patients, and hospital environment. Restriction fragment length polymorphism (RFLP) analysis revealed genetic diversity of S. aureus isolates. Some clones were transmitted among nurses, doctors and patients. Our studies also demonstrate contamination of the hospital environment with S. aureus strains and there is a possibility that the patients acquire staphylococci from the environment. Moreover, we found that many medical staff workers were colonized with S. aureus and the transmission of these strains to patients is possible.

3

Entwurf zu einem chemischen Apparate mittelst welchem das Miasma der asiatischen Cholera... aufgefangen... und die Krankheit ... geheilt werden kann

72%

Matejak M., Dobrowolska E.

Annals of Warsaw University of Life Sciences - SGGW. Forestry and Wood Technology

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2012

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tom 79

4

Detection of methicillin resistance in hospital environmental strains of coagulase-negative staphylococci

72%

Nowak T., Balcerczak E., Mirowski M., Szewczyk E. M.

Polish Journal of Microbiology

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2006

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tom 55

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nr 4

The aim of this study was to evaluate methicillin resistance detection methods currently used when studying coagulase-negative staphylococci (CoNS). The resistance to oxacillin of 142 strains from seven species of CoNS isolated from the Intensive Care Unit environments was tested. The methods used were: disc diffusion test with cefoxitin (FOX₃₀) and oxacillin (OX₁), oxacillin agar screen test with 6 mg/l of oxacillin (MHOXA), latex test for PBP2a (LA) and detection of mecA via PCR. One hundred and one isolates were methicillin-resistant in at least one of methods used, but only 74 were mecA -positive. Of the 68 mecX-negative strains: two were positive by OX₁, the LA and MHOXA methods; three by the LA and MHOXA; and 22 only by OX₁, test. Most of these strains were from the novobiocin-resistant CoNS group. The results obtained for all tested strains using FOX₃₀ showed complete concordance with the presence of the mecA gene.

5

Cytotoxicity of Aspergillus fungi isolated from hospital environment

58%

Gniadek A., Macura A., Gorkiewicz M.

Polish Journal of Microbiology

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2011

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tom 60

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nr 1

The majority of mycotoxins produced by Aspergillus fungi are immunosuppressive agents, and their cytotoxicity may impair defense mechanisms in humans. The objective of the study was evaluation of the cytotoxicity of fungi isolated from an environment where inpatients with impaired immunity were present. The materials comprised 57 fungal strains: Aspergillus fumigatus, Aspergillus niger. Aspergillus ochraceus, Aspergillus flavus, Aspergillus versicolor and Aspergillus ustus isolated from hospital rooms in Cracow. The cytotoxicity of all the strains was evaluated using the MTT test (3-(4,5-dimethylthiazol-2-yl) 2,5 diphenyltetrazolium bromide). To emphasize the differences in cytotoxicity among the particular strains, variance analysis (ANOVA) and Tukey's difference test were used. Out of 57 Aspergillus strains tested, 48 (84%) turned out to be cytotoxic. The cytyotoxicity was high (+++) in 21 strains, mainly in A. fumigatus. The least cytotoxic were A. niger fungi, this being statistically significant (p<0,05). To protect a patient from the adverse effects of mycotoxins, not only his or her immunity status should be evaluated but also the presence of fungi in hospital environment and their cytotoxicity should be monitored (possible exposure).

6

BOX-PCR is an adequate tool for typing of clinical Pseudomonas aeruginosa isolates

58%

Wolska K., Kot B., Jakubczak A., Rymuza K.

Folia Histochemica et Cytobiologica

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2011

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tom 49

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nr 4

7

Bacterial aerosol in Silesian hospitals: preliminary results

58%

Pastuszka J. S., Marchwinska-Wyrwal E., Wlazlo A.

Polish Journal of Environmental Studies

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2005

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tom 14

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nr 6

The concentration levels of airborne bacteria were measured in clinical/hospital rooms in Upper Silesia, Poland, in buildings of varying conditions. It was found that the typical level of bacterial aerosol concentration is about 10³ CFUm⁻³ in clinical outpatient rooms and ranges from 10² CFUm⁻³ to 10³ CFUm⁻³ in hospitals, depending on the number of occupants and physical quality of the building. The increased level of the airborne bacteria in patient rooms resulting from bed-making was noticed. The Staphylococcus/Micrococcus group was a dominating part of the bacteria in studied hospitals/clinic air, contributing together 58-78% of the total bacteria concentration, confirming that detected airborne bacteria mainly originated from human organisms. The size distributions of bacterial aerosol in naturally ventilated rooms have peaks in the size range between 1.1 and 3.3 μm while in the mechanically ventilated hospital rooms with HVAC the peak appears in the diameter range from 3.3 μm to 4.7 μm.

Ograniczanie wyników

Staphylococcus cohnii - resident of hospital environment: cell-surface features and resistance to antibiotics

Clonal analysis of Staphylococcus aureus strains isolated in Obstetric-Gynaecological Hospital

Entwurf zu einem chemischen Apparate mittelst welchem das Miasma der asiatischen Cholera... aufgefangen... und die Krankheit ... geheilt werden kann

Detection of methicillin resistance in hospital environmental strains of coagulase-negative staphylococci

Cytotoxicity of Aspergillus fungi isolated from hospital environment

BOX-PCR is an adequate tool for typing of clinical Pseudomonas aeruginosa isolates

Bacterial aerosol in Silesian hospitals: preliminary results