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The paper presents the statistical calculations of correlation coefficients (r) between the concentrations of various hormones in peripheral and ovarian venous plasma of hypo- and hyperthyroid, experimental cyst-bearing gilts. The experiment was carried out on 70 gilts randomly divided into the following six groups (10-16 heads in each): control, hypo- and hyperthyroid gilts (oral application of methylthiouracyl and injections of L-thyroxine) and gonadotropine (PMSG and hCG) treated eu- hypo- and hyperthyroid animals. The experiment started on the 2nd day of estrous cycle. Gonadotropins were applied starting from the 15th day of the cycle for 10 consequtive days. Peripheral venous blood samples were collected several times during the experiment by cannulation of the v. jugularis. On the 25th day of the experiment ovarian blood was collected after laparotomy by cannulation of uterovarian veins and again 2-3 days later (cannulae exposed on back of animals). The gilts were slaughtered on the 2nd - 3rd day after laparotomy. Ovaries were excised, inspected and the number of cysts, follicles and corpora lutea was counted. In several samples of peripheral and ovarian plasma of each gilt, the concentrations of luteinizing hormone (LH), prolactin (PRL), estrone (E,), estradiol- 17p (E2), progesterone (P4), androstenedione (A4), testosterone (T) and Cortisol (CI) were analysed by RIA procedures. The correlation coefficients (r) were calculated between concentrations of various hormones in both kinds of plasma in suitable pairs of individual animals of all groups as well as between their relations to the number of ovarian structures. Hypothyroid gilts evidenced correlation of the level of T to E1 and E2 but hyperthyroid animals - correlation of the level of A4 to P4, LH, PRL, Cl and PRL to P4 and LH. The euthyroid gilts after gonadotropine treatment showed interrelation of the concentration of PRL to LH and E, to E2, T and PRL. Hypothyroid and gonadotropine treated gilts evidenced numerous correlations, namely: the level of E1 to E2 and T and A4 to CI, E2, P4, P4 to PRL and T to LH. Hyperthyroid gilts treated with gonadotropins exhibited correlation of E, to T and A4 to CI. The number of cysts correlated mostly with the concentration of E1, E2, P4 and CI. The follicles showed only singular interrelations with the level of E1, P4, T, LH, PRL and Cl. The number of corpora lutea correlated only with the level of E1 and LH.
Objectives: Molecular mechanism of carcinogenesis associated with high risk (HR) type HPV is related to the activity of virus oncoproteins E5, E6 and E7. Their task is to bring the cells to a state enabling synthesis of viral DNA, copying viral particles and promotion of uncontrolled cell growth. Proliferative factors of a feminine genital tract epithelium are also sex hormones — estradiol and progesterone. Steroids influence the transcription of genes involved in cell cycle, acting through specific nuclear receptors (ER and PR). The aim of this study was evaluation of the effect of selected concentrations of 17ß-estradiol and progesterone on survival and proliferation of HeLa line cells. Material and methods: The study was done on a transformed HeLa cell line containing integrated genome HPV of type 18. The lines were incubated in the presence of 17ß-estradiol at the concentrations of lx10-4M, lx10-5M, lx10-7M, lx10-8M. Cell survival was determined with the use of 0.5% of water solution of toluidine blue and the cell proliferation rate were evaluated with the use of BrdU Labelling and Detection Kit and the method ELISA (Roche). Results: The obtained results point to 10% toxic influence on HeLa line cells of 17ß-estradiol at high concentrations after 72 h of incubation. The influence of the hormone on proliferation rate was diversified depending on the hormone concentration and time.
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