Wyniki wyszukiwania

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Zastosowanie technik hodowli przestrzennych [3D] w badaniach biologii gruczolu mlekowego bydla

100%

Kozlowski M., Motyl T.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 11 Supl.

1417-1420

The mammary gland has been extensively studied for over a century. However, the major contribution to our current knowledge of mammary gland biology has been made mostly by studying the mammary gland of humans and rodents, while ruminant models have lagged behind. During the last years, a variety of in vitro approaches have been employed to understand the regulation of mammary gland growth and morphogenesis during different developmental and physiological stages. There are only a few immortalized cell lines of bovine mammary gland epithelium. Unfortunately only three of them are IGF and EGF sensitive, which is very important in mammary gland development in vivo. Monolayer culture models are easy and convenient to set up but they lack the three-dimensional microenvironment of intact tissue. This review describes the three-dimensional (3-D) cell culture system, which has recently been widely used. Bovine primary mammary epithelium cell cultures and some immortalized cell lines plated on an extracellular matrix form functional acini-like structures. This model mimics organization and differentiation of an intact tissue and can be very useful in the investigation of bovine mammary gland biology.

2

Trojwymiarowe hodowle komorek nablonka gruczolu sutkowego jako model badawczy procesu roznicowania

86%

Gajewska M., Kozlowski M., Motyl T.

Postępy Biologii Komórki

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2010

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tom 37

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nr 1

121-135

3

Rola autofagii w mammogenezie gruczolu mlekowego krow na modelu mammosfer

86%

Sobolewska A., Gajewska M., Motyl T.

Medycyna Weterynaryjna

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2010

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tom 66

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nr 04

222-227

Progress in studies concerning the process of mammogenesis have been stimulated by the development of the three-dimensional (3D) culture systems, which enable mammary epithelial cells to form structures mimicking the alveoli of mammary gland in vivo. Mammary epithelial cells (MECs) supported on a laminin-rich extracellular matrix (ECM) form 3D acinar structures - mammospheres - which mature to form polarized and functional monolayers surrounding a lumen and have the ability to produce milk proteins. These structures develop an axis of apico-basal polarity, subsequently become unresponsive to proliferative signals, and finally a bona fide lumen is formed by cavitation, involving the removal of centrally localized cells via multiple cell death processes. Lumen formation is associated with the selective apoptosis of centrally located cells. Autophagy, which is a process responsible for maintaining cell homeostasis, also seems to be crucial in mammary gland development and remodeling. This review describes the role of autophagy in the formation of acinar structures by mammary epithelial cells. Studies on MECs from different species (human, mouse, cow) cultured on Matrigel™ have shown the protective role of autophagy in centrally located cells of differentiating mammospheres. Autophagy seems to be the cells’ first response to the lack of contact with ECM, which in consequence leads to apoptotic cell death, anoikis, and lumen formation in developing alveoli.

Ograniczanie wyników

2 Medycyna Weterynaryjna

1 Postępy Biologii Komórki

3 Motyl T.

2 Gajewska M.

2 Kozlowski M.

1 Sobolewska A.

2 2010

1 2007

Zastosowanie technik hodowli przestrzennych [3D] w badaniach biologii gruczolu mlekowego bydla

Trojwymiarowe hodowle komorek nablonka gruczolu sutkowego jako model badawczy procesu roznicowania

Rola autofagii w mammogenezie gruczolu mlekowego krow na modelu mammosfer