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Total and high molecular weight adiponectin levels in the rat model of post-myocardial infarction heart failure

100%

Kalisz M., Baranowska B., Wolinska-Witort E., Maczewski M., Mackiewicz U., Tulacz D., Gora M., Martynska L., Bik W.

Journal of Physiology and Pharmacology

2015

tom 66

nr 5

DFF40-CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis

100%

Widlak P.

Cellular and Molecular Biology Letters

2000

tom 05

nr 3

Sequential cleavage of genomic DNA into large-scale DNA fragments of 50-300-kb, followed by formation of mono- and oligonucleosomal DNA fragments, is a biochemical hallmark of programmed, cell death (apoptosis). The endonuclease DFF40/CAD mediates regulated internucleosomal DNA fragmentation and chromatin condensation in cells undergoing apoptosis. DFF40 hypersensitive sites were detected in purified HeLa cell nuclei, and excision of 50-kb DNA fragments preceded formation of oligonucleosomal DNA ladders in nuclei treated with the nuclease. Topoisomerase II, but not topoisomerase I, stimulates DFF40 activity on plasmid DNA substrates. This suggests that interactions of DFF with the nuclear matrix-bound topoisomerase II may be involved in formation of DFF40 hypersensitive sites.

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil

61%

Abou Dobara M., El-Sayed A., El-Fallal A., Omar N.

Polish Journal of Microbiology

2011

tom 60

nr 1

Optimizing production of α-amylase production by Thermoactinomyces vulgaris isolated from Egyptian soil was studied. The optimum incubation period, temperature and initial pH of medium for organism growth and enzyme yield were around 24 h, 55°C and 7.0, respectively. Maximum α-amylase activity was observed in a medium containing starch as carbon source. The other tested carbohydrates (cellulose, glucose, galactose, xylose, arabinose, lactose and maltose) inhibited the enzyme production. Adding tryptone as a nitrogen source exhibited a maximum activity of α-amylase. Bactopeptone and yeast extract gave also high activity comparing to the other nitrogen sources (NH₄Cl, NH₄NO₃, NaNO₃, KNO₃, CH₃CO2 NH₄). Electrophoresis profile of the produced two α-amylase isozymes indicated that the same pattern at about 135145 kDa under different conditions. The optimum pH and temperature of the enzyme activity were 8.0 and 60°C, respectively and enzyme was stable at 50°C over 6 hours. The enzyme was significantly inhibited by the addition of metal ions (Na⁺, Co²⁺ and Ca²⁺) whereas Cl⁻ seemed to act as activator. The enzyme was not affected by 0.1 mM EDTA while higher concentration (10 mM EDTA) totally inactivated the enzyme.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology

1 Polish Journal of Microbiology

1 Abou Dobara M.

1 Baranowska B.

1 Bik W.

1 El-Fallal A.

1 El-Sayed A.

1 Gora M.

1 Kalisz M.

1 Mackiewicz U.

1 Maczewski M.

1 Martynska L.

1 Omar N.

1 Tulacz D.

1 Widlak P.

1 Wolinska-Witort E.

1 2015

1 2011

1 2000

Wyniki wyszukiwania

Total and high molecular weight adiponectin levels in the rat model of post-myocardial infarction heart failure

DFF40-CAD hypersensitive sites are potentially involved in high molecular weight DNA fragmentation during apoptosis

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil