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The content of alkaloids and flavonoids and the yield of herb were analyzed in greater celandine cultivar ‘Cynober’ during six following phases: spring rosette formation, the beginning of flowering, full bloom, green fruit, seed harvest, fall rosette formation. Yield of celandine herb was different at the investigated phases and in years of cultivation. The highest yield of herb was observed at the beginning of flowering, then a decrease was noticed, up to the phase of seed harvest, when the yield grown up. The lowest yield of celandine herb was obtained in last phase – fall rosette formation, except 2011 when the lowest yield was in spring rosette formation. The average content of alkaloids was the highest in phase of green fruit (1.097%), while in 2012, the highest content was reached in phase of fall rosette formation – 1.200%. The lowest content of alkaloids was obtained in herb of the beginning of flowering (0.608%) in both years. The mean content of flavonoids was from 0.310% (the beginning of flowering) to 0.522% (seed harvest) and was the same in both years. The stable high content of total alkaloids and flavonoids and individual alkaloids was noticed in phase of fall rosette. Our results suggest that seed maturity is the best time for celandine herb harvest regarding the herb yield and content of alkaloids and flavonoids.
During the years 2007–2008 the content of flavonoids and polyphenolic acids in inflorescences of Helichrysum arenarium (L.) Moench was analyzed in specimens from natural stands and plantations. Plants cultivated in experimental plots originated from in vitro plantlets. The amount of flavonoids in inflorescences from experimental plots was higher and attained its maximum at 1.19% compared with 0.84% observed for plants from natural stands. In herbal material collected in 2008 the content of flavonoids was lower. The tendency was not observed for polyphenolic acids. Within the study period their amount ranged over the interval 0.74–2.82%. It was also found that dry mass of inflorescences from natural stands was higher than that for cultivated plants by 30.9%. In contrast, the density of generative shoots was 5.2-fold higher in natural stands. It was shown that Sandy Everlasting plantations could yield up to 3.4-fold more herbal material which is of a better quality (contains more biologically active compounds) than that from natural stands.
Within the Ocimum genus there occur about 200 species in different varieties and forms. They differ in the essential oil content and quality, as well as in many morphological features. The studies conducted in the years 2008–2009 were aimed at defining the effect of basil cultivar (‘Kasia’ and ‘Wala’), as well as of the differentiated doses of CaCO3 (6 or 12 g·dm-3 of substratum) upon the biological value and chemical composition of the oil in the examined plants, grown in pots filled with transitory peat. A significant interaction was reported between the examined cultivar and CaCO3 dose upon the yield of fresh basil plant weight. The highest yield (172.3 g·plant-1) was obtained from plants of ‛Kasia’ cultivar, when a higher dose of CaCO3 was applied (12 g·dm-3 substratum). Lack of significant effect of calcium carbonate dose was reported upon the yield of fresh basil plant weight. In the examined objects similar fresh weight yield values were obtained. At a higher dose of calcium carbonate the unit plant weight equaled 165.9 g·plant-1, and at a lower dose of CaCO3 it was 160.2 g·plant-1. The percentage of dry matter significantly depended on the cultivar. Basil of ‘Wala’ cultivar had higher dry matter content, (32.0%) compared to ‘Kasia’ (25.6%). Both the cultivar and calcium carbonate dose significantly affected the vitamin C contents in fresh basil herb. Increasing the CaCO3 dose from 6 g·dm-3 to 12 g·dm-3 caused the increase of vitamin C concentration by 12.6%. Higher essential oil contents was characteristic of the herb of ‘Kasia’ plants – 1.33% compared to ‘Wala’ cultivar, in whose herb 1.03% of oil was determined. The qualitative composition of isolated oil depended on the examined factors. The predominating compounds were: linalool, 1.8-cineole, geranyl, D germacrene, γ-cadinene, Epi-α -cadinole. A certain differentiation was found in the contents of particular compounds in the essential oils of ‘Kasia’ and ‘Wala’ plants, as the effect of calcium nutrition.
The studies conducted in 2007–2008 were aimed to assess how the developmental stage of common oregano affected the quality and quantity of the herb yield. The field experiment was established with the use of random blocks in four repetitions. Oregano was grown from seeding produced in a greenhouse. Herbage harvest of one-year-old plants was conducted before flowering, in budding phase, at the beginning of flowering and in the full flowering phase. The yield of fresh and air dry herb yield and then the contents of essential oil was determined. The oil was chemically analyzed with the use of GC/MS method. It was demonstrated that the best term for oregano raw material harvest was the full flowering stage of the plants. In this period the yields of fresh and air dry weights as well as the essential oil contents were the greatest. The oil from the herb collected in the initial phase of flowering had the largest number of components, whereas the oil obtained in the budding phase had the smallest. The predominant components of oregano oil were: sabinene, germacrene D, E-caryophyllene, (Z)-β-ocimene and γ-terpinene. The examined oil also contained phenol compounds: thymol and carvacrol – highly bactericidal and fungicidal substances.
The object of experiments carried out in 2007 and 2008 were plants of lemon balm (Melissa officinalis L.) which were at that time in the 1st, 3rd and 4th year (2007) and in the 2nd, 4th and 5th year (2008) of growing. The soil from plots on which the plants were grown was studied as well. The yield of fresh and dry matter of the herbal raw material as well as the essential oil content in the raw material were determined. Furthermore, the soil phytotoxicity and phenolic compounds content in the soil were studied. Maximum yield of the fresh and dry weight of the herbal raw material were obtained in the 3rd and 4th year of plant cultivation and it was maintained on an unchanged level in the 4th and 5th year of growing. As the time of lemon balm growing was elongated, the increase of essential oil yield was observed. Phytotoxicity of soil occurred seasonally – in spring and in autumn and did not depend on the year of growing duration. On the other hand, the content of phenolic compounds in the soil increased gradually with the long-term cultivation. However, the phenolic compounds did not accumulate in such amount as to exert a negative effect on the plants.
The experiment was carried out in Slovenia in 2002-03 and 2004-05. The experimental design was a randomized completed block, with four replicates. In the summer after pea harvesting, 10, 20 and 30 kg of Italian ryegrass ha-1 were drilled into minimally cultivated pea stubble, in which, on average, 12.5 pea grains m-2 were left among crop residues. The ryegrass/pea mixtures in our experiments took up the soil mineral nitrogen the most efficiently and accumulated the highest amount of nitrogen in the autumn herb­age yield, at a seeding rate of 30 kg drilled Italian ryegrass. The differences among treatments regarding 0-60 cm soil N03-N and NH4-N contents were highest at the first cut and decreased until March of the next year. In order to achieve efficient early and high N uptake of mineralized nitrogen, it is not recommended to lower the sowing rates of Italian ryegrass for drilling into pea stubble to reduce the high cost of catch crop seeds.
The effect of various forms of nitrogen fertilizer and dates of application on Ocimum amercianum yield and essential oil (ton/feddan, 1 feddan = 4200 m2) was studied in two successive seasons. In both seasons the total yields of fresh and dry herb after the application of ammonium sulfate surpassed that of ammonium nitrate and urea. Dividing the full amount of fertilizer into two equal portions gave the highest yields in most cuts. Nitrogen fertilizers affected the percentage of essential oil in most cuts. The highest oil percentage was obtained after the application of ammonium nitrate and ammonium sulfate in both cuts in the first season, respectively, and urea in the second season. Early addition of the full amount of fertilizer was more effective in increasing oil percentage as compared to other two dates of application. Ammonium sulfate in the first season and urea in the second one produced the highest total oil yields either per plant or per feddan. One application of the full amount of ammonium nitrate at the early date produced the highest total percentage of monoterpenic compounds as compared to ammonium sulfate and urea. One application the full amount of nitrogen fertilizer at the early date was superior to other treatments. The opposite was true for sesquiterpenes. Oxygenated compounds showed the same trend of monoterpenic compounds. Thus, the application of ammonium sulfate at the rate of 60 kg N/fed/season at two equal portions in each cut could be recommended for maximizing herb, leaves and oil yields of Ocimum amercianum, L. var. pilosum plants.
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A two-factorial experiment was conducted in 2012-2014 in a greenhouse owned by the Department of Horticulture at the University of Warmia and Mazury in Olsztyn. The experiment had a randomised block design with three replicates. The first experimental factor were six botanical varieties of basil: sweet basil, Thai ‘Siam Queen’ basil, Greek ‘Minette’ basil, purple basil, lemon basil and cinnamon basil. The second experimental factor was container size and the number of plants per pot: 0.7 dm3 container with one plant per pot, 3.0 dm3 container with one plant per pot, 3.0 dm3 container with four plants per pot. In successive years of the three-year experiment, between 22 and 24 February, containers filled with organic substrate (sphagnum peat) were placed on movable tables in the greenhouse, depending on pot size (0.7 and 3.0 dm3). Once-over harvest of basil herbage was carried out between 17 April and 7 May. The plants were cut at 5 cm above the substrate surface. Total yield and marketable yield were identical because no mechanical damage to leaves or symptoms of leaf diseases were observed. The content of dry matter, total sugars and L-ascorbic acid in basil herbage was determined. Basil ecotype had a significant effect on leaf greenness index, fresh herbage yield, dry herbage yield, and on the content of dry matter and total sugars. Growing basil plants in 3.0 dm3 containers, with four plants per pot, contributed to a significant decrease in fresh and dry herbage yields, in comparison with the remaining treatments.
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