Wyniki wyszukiwania

1

Comparison of light microscopy and polymerase chain reaction for the detection of haemoparasites in cattle in Nigeria

100%

Happi A. N., Osifade O., Oluniyi P. E., Ogunro B. N.

Acta Parasitologica

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2020

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tom 65

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nr 1

2

First report of Hepatozoon (Apicomplexa: Adeleorina) from king ratsnakes (Elaphe carinata) in Shanghai, with description of a new species

84%

Han H., Wu Y., Dong H., Zhu S., Li L., Zhao Q., Wu D., Pei E., Wang Y., Huang B.

Acta Parasitologica

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2015

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tom 60

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nr 2

3

Hemoparasites and fleas of shrews and rodents from Alaska

84%

Laakkonen J., Henttonen H., Hastriter M. W., Niemimaa J., Jarrell G. H.

Acta Parasitologica

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2002

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tom 47

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nr 3

We examined 49 Sorex cinereus, 25 S. tundrensis, 15 S. hoyi, two S. monticolas, two S. ugyunak, 19 Microtus pennsylvanicus, 45 M. oeconomus, 60 M. miurus, 13 Clethrionomys rutilas, six Synaptomys borealis collected from central and northern Alaska in August 2000 for blood parasites. Bartonella sp. was found in blood smears of 2% of S. cinereus, and Trypanosoma sp. in blood smears of 7% M. oeconomus. Meronts of Hepatozoon sp. were detected in lungs of 2% of M. oeconomus and 1.5% of M. miurus. Of possible vectors of blood parasites, species of fleas collected included Amalaraeus dissimilis dissimilis from M. miurus, Peromyscopsylla ostsibirica from S. tundrensis and M. oeconomus, and Corrodopsylla curvata curvata from S. cinereus.

4

Detection of Theileria equi in spleen and blood of asymptomatic piroplasm carrier horses

67%

Ribeiro I. B., Camara A. C. L., Bittencourt M. V., Marcola T. G., Paludo G. R., Soto-Blanco B.

Acta Parasitologica

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2013

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tom 58

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nr 2

This study aimed to determine whether asymptomatic horses naturally infected with Theileria equi retain infected erythrocytes in the spleen and whether the presence of the hemoparasite in this organ is associated with parasitemia. We collected samples from 25 adult horses without clinical signs of any disease. From each animal, we collected whole blood samples from the jugular vein and a splenic puncture blood sample. All samples were submited to blood cell counts and detection of Theileria or Babesia. DNA extraction and PCR were performed in all samples for identification of piroplasm infection (T. equi and B. caballi). From the 25 horses evaluated for piroplasm detection by PCR, seven horses (28%) were positive in jugular vein blood but negative in splenic blood samples, five horses (20%) were positive in splenic blood samples but negative in jugular vein blood samples, and 13 horses (52%) were positive in both jugular vein and splenic blood samples. The hematological evaluation revealed anemia in 13 of 25 (52%) infected horses, lymphopenia in five (20%), neutrophilia in two (8%), neutropenia in one (4%), and thrombocytopenia in one (4%) infected horse. The present study demonstrated that several (20%) of the asymptomatic piroplasm carrier horses did not show parasitemia, but show infected erythrocytes in the spleen.

5

Apicomplexa primers amplify Proteromonas (Stramenopiles, Slopalinida, Proteromonadidae) in tissue and blood samples from lizards

67%

Maia J. P. M. C., Gomez-Diaz E., Harris D. J.

Acta Parasitologica

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2012

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tom 57

|

nr 4

Microscopy has traditionally been the most common method in parasitological studies, but in recent years molecular screening has become increasingly frequent to detect protozoan parasites in a wide range of vertebrate hosts and vectors. During routine molecular screening of apicomplexan parasites in reptiles using the 18S rRNA gene, we have amplified and sequenced Proteromonas parasites from three lizard hosts (less than 1% prevalence). We conducted phylogenetic analysis to confirm the taxonomic position and infer their relationships with other stramenopiles. Although our phylogeny is limited due to scarcity of molecular data on these protists, our results confirm they are closely related to Proteromonas lacertae. Our findings show that unexpected parasites can be amplified from host samples (blood and tissue) using general procedures to detect hemoparasites, and stress that positive PCR amplifications alone should not be considered as definitive proof of infection by particular parasites. Further validation by sequence confirmation and thorough phylogenetic assessment will not only avoid false positives and biased prevalence estimates but also provide valuable information on the biodiversity and phylogenetic relationships of other parasitic organisms. More generally, our results illustrate the perils of general diagnosis protocols in parasitological studies and the need of cross-validation procedures.

6

Does habitat structure affect body condition of nestlings? A case study with woodland Great Tits Parus major

67%

Sanchez S., Cuervo J. J., Moreno E.

Acta Ornithologica

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2007

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tom 42

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nr 2

200-204

Ograniczanie wyników

Comparison of light microscopy and polymerase chain reaction for the detection of haemoparasites in cattle in Nigeria

First report of Hepatozoon (Apicomplexa: Adeleorina) from king ratsnakes (Elaphe carinata) in Shanghai, with description of a new species

Hemoparasites and fleas of shrews and rodents from Alaska

Detection of Theileria equi in spleen and blood of asymptomatic piroplasm carrier horses

Apicomplexa primers amplify Proteromonas (Stramenopiles, Slopalinida, Proteromonadidae) in tissue and blood samples from lizards

Does habitat structure affect body condition of nestlings? A case study with woodland Great Tits Parus major