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1

Bone marrow morphology during haematopoietic stem cell mobilization with G-CSF in mice

100%
Barcew K., Kacinska E., Marchlewicz M., Wiszniewska B., Machalinski B.
Folia Morphologica
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2004
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tom 63
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nr 1
The aim of the work was to examine the morphology of the bone marrow of mice during stimulation with G-CSF. Experimental Balb C mice were daily injected subcutaneously with 250 μg/kg b.w. G-CSF (Neupogen). After 2, 4 and 6 days of the experiment femurs were obtained for morphological study. On day 2 of the mobilization the amount of haematopoietic cells in the bone marrow increased and dilatation of the sinusoids was observed. Only single leukocytes were observed in the lumen of the vessels. There were numerous leukocytes in the lumen of the sinusoids on day 4 of the mobilization. The morphology of the bone marrow on day 6 was similar to that of the control. Mobilization of mice with G-CSF resulted in migration of haematopoietic cells from the bone marrow and the process is most pronounced on day 4.
2

The biology of the human hematopoietic stem and progenitor cells

100%
Ratajczak M. Z.
Folia Histochemica et Cytobiologica. Supplement
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1997
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tom 35
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nr 2
3

A comparative gene-expression analysis of CD34plus hematopoietic stem and progenitor cells grown in static and stirred culture systems

100%
Li Q., Liu Q., Cai H., Tan W.
Cellular and Molecular Biology Letters
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2006
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tom 11
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nr 4
Static and stirred culture systems are widely used to expand hematopoietic cells, but differential culture performances are observed between these systems. We hypothesize that these differential culture outcomes are caused by the physiological responses of CD34+ hematopoietic stem and progenitor cells (HSPCs) to the different physical microenvironments created in these culture devices. To understand the genetic changes provoked by culture microenvironments, the gene expression profiling of CD34+ HSPCs grown in static and stirred culture systems was compared using SMART-PCR and cDNA arrays. The results revealed that 103 and 99 genes were significantly expressed in CD34+ cells from static and stirred systems, respectively. Of those, 91 have similar levels of expression, while 12 show differential transcription levels. These differentially expressed genes are mainly involved in anti-oxidation, DNA repair, apoptosis, and chemotactic activity. A quantitative molecular understanding of the influences of growth microenvironments on transcriptional events in CD34+ HSPCs should give new insights into optimizing culture strategies to produce hematopoietic cells.
4

The effect of stem cell mobilisation with granulocyte colony-stimulating factor on the morphology of the haematopoietic organs in mice

67%
Barcew K., Paczkowska E., Dabkowska E., Baskiewicz-Masiuk M., Marchlewicz M., Domanski L., Sagan L., Wiszniewska B., Machalinski B.
Folia Morphologica
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2007
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tom 66
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nr 1
The cellular mobilisation of mice with granulocyte colony-stimulating factor (G-CSF) results in an egress of haematopoietic stem/progenitor cells from the bone marrow and an increase in their level in the peripheral blood. While the mobilisation process with different agents is widely studied, little is known about the morphology of the murine haematopoietic organs during the mobilisation. The purpose of this study was to examine the morphology of the bone marrow, spleen and liver in mice mobilised with G-CSF. To address this issue mice were injected subcutaneously with G-CSF for 6 consecutive days. Morphological analysis revealed an increase in the number of mature neutrophils close to the wall of sinusoids in the bone marrow as well as hypertrophy of the red pulp in the spleen. At the same time no morphological changes were noticed in the livers of G-CSF-mobilised mice. In conclusion, G-CSF induces discrete ultrastructural changes in the bone marrow, which intensify the transendothelial traverse of haematopoietic stem and progenitor cells from it. The changes in the spleen are related to repopulation of this organ by mobilised early haematopoietic cells circulating in the peripheral blood. We also noticed that the process of migration of haematopoietic cells from the bone marrow into the peripheral blood began on day 2 and was most pronounced on day 4 after stimulation with G-CSF.
5

Evidence that platelet-derived microvesicles may transfer platelet-specific immunoreactive antigens to the surface of endothelial cells and CD34plus hematopoietic stem-progenitor cells - implication for the pathogenesis of immune thrombocytopenias

67%
Majka M., Kijowski J., Lesko E., Gozdzik J., Zupanska B., Ratajczak M. Z.
Folia Histochemica et Cytobiologica
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2007
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tom 45
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nr 1
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