Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: heart development

Sortuj według:

Ogranicz wyniki do:

Expression of cellular retinoic acid-binding protein I and II (CRABP I and II) in embryonic mouse hearts treated with retinoic acid

100%

Stachurska E., Loboda A., Niderla-Bielinska J., Szperl M., Juszynski M., Jozkowicz A., Dulak J., Ratajska A.

Acta Biochimica Polonica

2011

tom 58

nr 1

Cellular retinoic acid binding proteins are considered to be involved in retinoic acid (RA) signaling pathways. Our aim was to compare the expression and localization of cellular retinoic acid binding proteins I and II (CRABP I and II) in embryonic mouse hearts during normal development and after a single teratogenic dose of RA. Techniques such as real-time PCR, RT-PCR, Western blots and immunostaining were employed to examine hearts from embryos at 9-17 dpc. RA treatment at 8.5dpc affects production of CRABP I and II in the heart in the 48-h period. Changes in expression of mRNA for retinaldehyde dehydrogenase II (Raldh2), Crabp1 and Crabp2 genes also occur within the same time window (i.e. 10-11dpc) after RA treatment. In the embryonic control heart these proteins are localized in groups of cells within the outflow tract (OT), and the atrioventricular endocardial cushions. A gradient of labeling is observed with CRABP II but not for CRABP I along the myocardium of the looped heart at 11 dpc; this gradient is abolished in hearts treated with RA, whereas an increase of RALDH2 staining has been observed at 10 dpc in RA-treated hearts. Some populations of endocardial endothelial cells were intensively stained with anti-CRABP II whereas CRABP I was negative in these structures. These results suggest that CRABP I and II are independently regulated during heart development, playing different roles in RA signaling, essential for early remodeling of the heart tube and alignment of the great arteries to their respective ventricles.

Correlation of heart external dimensions with body external dimensions of human foetuses

100%

Nowak D., Bozilow W., Walek S.

Folia Morphologica

2003

tom 62

nr 1

In an evaluation of foetal hearts in II and III pregnancy semester, correlation with exterior foetal dimensions should be taken into consideration. It is interesting also if all exterior heart dimensions are correlated at the same level with overall body dimensions. The subject of examination was 204 foetuses of both sexes, including 106 males and 98 females, in 5th and 6th month of foetal life. Foetuses were fixed for minimum 3 months in 9% formalin solution. They were taken from natural abortions, without exterior features of developmental malformations. Examined material was evaluated in month groups of morphological age. The number of foetuses in different groups was various. Exterior heart dimensions were considered: height, width, heart depth, and also heart circumference in coronary sulcus (atrial-ventricular). Exterior dimensions of foetuses were: vertex-plantare, vertex-tubulare. All exterior dimensions of heart are closely and strongly correlated with both exterior dimensions of foetuses.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Folia Morphologica

1 Bozilow W.

1 Dulak J.

1 Jozkowicz A.

1 Juszynski M.

1 Loboda A.

1 Niderla-Bielinska J.

1 Nowak D.

1 Ratajska A.

1 Stachurska E.

1 Szperl M.

1 Walek S.

1 2011

1 2003

Wyniki wyszukiwania

Expression of cellular retinoic acid-binding protein I and II (CRABP I and II) in embryonic mouse hearts treated with retinoic acid

Correlation of heart external dimensions with body external dimensions of human foetuses