Wyniki wyszukiwania

1

Prevalence of EBV genotypes in Polish, Taiwanese and Arabic healthy students and association between genotypes and 30-bp deletion in the LMP-1 gene phylogenetic analysis

100%

Polz D., Podsiadlo L., Stec A., Polz-Dacewicz M.

Polish Journal of Microbiology

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2014

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tom 63

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nr 1

The aim of this study was to compare the prevalence of EBV genotype and del-LMP-1 in saliva from Polish, Taiwanese and Arabic healthy students. The study group consisted of 56 healthy students; 24 of them Polish, 25 Taiwanese, and 7 Arabic. Typing was carried out using PCR with EBNA-2 primers. A detection of LMP-1 variants was also performed using PCR. EBV DNA was detected in 22 investigated samples (39.3%). Type 1 of the virus was dominant in both Polish and Taiwanese group. Among 62.5% Tai wanese with EBV 1 and 55.6% Polish detected EBV with 30-bp deletion in LMP-1 gene.

2

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Dietary fats and the risk of oxidative stress in a group of apparently healthy women - a short report

100%

Witkowska A. M., Zujko M. E.

Polish Journal of Food and Nutrition Sciences

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2013

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tom 63

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nr 2

The study aimed to determine associations between diet’s composition and serum antioxidant potential in 29 women aged 19–22 years. The participants completed self-report questionnaires concerning health condition, body measures, dietary habits and supplements taken, and 3-day detailed diet records. Fasting blood samples were collected to assess total antioxidant status (TAS) and ferric reducing antioxidant power (FRAP) in the serum. The women reported good health condition and lean body. The data concerning the TAS and FRAP methods demonstrated that the antioxidant potential was negatively correlated with saturated fat intake (r=-0.515 and r=-0.527, respectively), but not related to the intakes of protein, carbohydrate, mono- and polyunsaturated fats, cholesterol and antioxidant vitamins (vitamin C, β-carotene, α-tocopherol). The TAS antioxidant activity of the serum was signifi cantly lower in the top vs. bottom quartile of the saturated fat intake, which corresponds to a consumption of at least 29 g saturated fat vs. intake below 19.7 g. The FRAP value in the highest quartile of the saturated fat intake was also reduced and close to significance. This study shows that the studied group of young women exposed to diets, which contained high amounts of saturated fat, are prone to risk of oxidative stress.

3

The influence of age and gender on the latency of eye movement in healthy humans

100%

Jernajczyk W., Sobanska A., Czerwosz L., Szatkowska E.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 4

93-97

Standard measures of sleep recordings give differing results depending on the gender and they constantly change with age. Sleep latency increases, delta sleep decreases, and sleep tend to be shorter in duration and fragmented in middle-aged and elderly adults. The deterioration of sleep is observed earlier in men. In the 1980s, new measures called the Latency of Eye Movement (LEM) and the Mean Latency of Eye Movement (M-LEM) were proposed. Previous studies have shown that untreated patients with endogenous depression had the LEM and M-LEM shortened and that both indices get prolonged during treatment with antidepressants. On the other side, alcoholics in the abstinence period have LEM and M-LEM twice as long as healthy controls. In this study we set out to compare LEM and M-LEM in healthy humans according to the gender and age. The subjects of the study were 80 healthy volunteers: 40 males and 40 females, who were divided into 4 groups: females and males, below and above 40 years of age. In contrast to standard measures, our study did not reveal any significant changes of LEM or M-LEM due to the gender or age.

4

Melatonin in human volunteers: focus on nasal lavage level and its free radical scavenging properties with possibility to apply in food research - a short report

100%

Zielinski H., Mudway I., Lewczuk B., Przybylska-Gornowicz B., Kelly F. J.

Polish Journal of Food and Nutrition Sciences

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2007

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tom 57

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nr 2

215-219

The present paper is the first to report the results of the work carried out on daytime melatonin levels in paired plasma and nasal lavage fluid (NAL) samples obtained from healthy adult subjects. Lavage fluid was collected from 25 volunteers using a modified Foley catheter. Blood plasma was collected using normal procedure. The immunoreactive melatonin was present in all plasma samples and in 80% of the investigated NAL samples. Blood plasma melatonin level ranged from 0.98 pg/mL to 34.97 pg/mL however the level found in NAL was lower and ranged from 0.46 to 2.42 pg/ mL There was no correlation between paired plasma and nasal lavage melatonin concentration (r=0.02). Moreover, the superoxide dismutase activity (SOD) in NAL samples was determined and then the correlation coefficient between the melatonin concentration and EC-SOD activity was r= 0.18. The peroxyl radical trapping activity of 1 μmol/L melatonin was equivalent to 3 μmol/L Trolox. It has been concluded that nasal lavage fluid melatonin has limited utility in the field of melatonin research.

5

Occurrence of Candida spp. in healthy oral microbiota

100%

Kimsa L., Tokarska-Rodak M.

Health Problems of Civilization

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2020

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tom 14

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nr 2

Background. Candida is a strain of fungi that is found on the surface of healthy mucous membranes of the oral cavity. Yet, Candida may also be considered opportunistic microorganisms. The pathogenicity of Candida spp. depends on strain-specific characteristics including, invasiveness, adhesion, and production of biofilm abilities as well as the production of enzymes allowing the colonization of tissue. The aim of the paper was to evaluate the frequency of Candida spp. occurrence in the oral microbiota of healthy adults, and its relation with risk factors and colonization. Material and methods. This study consisted of 72 healthy participants (from the Lubelskie Voivodeship between 19 and 73 years of age). Medical history was collected to determine potential relationships to risk factors affecting oral microbiota (past oral cavity diseases, chronic diseases, and smoking). The participants who were qualified had no oral cavity infections during the study. The collected clinical samples (oral cavity swabs) were disseminated on the chromogenic base for isolation and differentiation of Candida spp. Results. The frequency of Candida spp. occurrence was at the level of 63.8%. C. albicans was the most frequently identified species (56.5%). C. glabrata (45.6%) and C. tropicalis (23.9%) were identified less often. In 21.7% of swabs, two species of Candida were identified and in 2.2% of swabs three species identified. The most common colonization risk factor was associated with smoking (32.6%). Conclusions. Yeasts of Candida species are part of the healthy microflora of the oral cavity in people of different ages and may occur as single species or coexist with other species. The coexistence of chronic diseases, propensity for oral cavity infections and smoking significantly influence the Candida colonization. This may result in future clinical consequences, for example in cases of immunodepression.

6

Assessment of the antioxidant effectiveness of alpha-lipoic acid in healthy men exposed to muscle-damaging exercise

100%

Zembron-Lacny A., Slowinska-Lisowska M., Szygula Z., Witkowski K., Stefaniak T., Dziubek W.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 2

139-143

The aim of this study was to compare the indices of glutathione antioxidant system and oxidative damage level in resistance trained and untrained subjects and to assess the antioxidant action of -lipoic acid in trained men exposed to muscle-damaging exercise. Thirteen trained and twenty untrained men (NT) participated in the comparative study. Then trained men were randomly assigned to TCON group (control) or TALA group (-lipoic acid, 600 mg . day-1, for 8 days) and performed isometric/isokinetic effort of quadriceps muscles. The study has shown the significantly higher erythrocyte levels of glutathione (GSH), glutathione reductase (GR) and glutathione peroxidase (GPx) in TCON than NT but no differences in plasma lipid peroxidation (TBARS) and protein carbonylation (PC). However, total thiol (TT) concentration was two-fold lower in TCON than NT group. -Lipoic acid variously influenced the post-exercise levels of GSH (+40%), GR (-24%) and GPx (+29%), but markedly reduced by over 30% the resting and post-exercise TBARS and PC in TALA compared with TCON. TT concentration significantly increased in TALA but it did not reach the high level which was found in untrained group. It is concluded that -lipoic acid supplementation diminishes oxidative damage. It does not abolish differences in glutathione antioxidant system between untrained and trained subjects but modulates a pro-antioxidant response to the muscle-damaging exercise.

7

Effect of 5-week moderate intensity endurance training on the oxidative stress, muscle specific uncoupling protein (UCP3) and superoxide dismutase (SOD2) contents in vastus lateralis of young, healthy men

80%

Majerczak J., Rychlik B., Grzelak A., Grzmil P., Karasinski J., Pierzchalski P., Pulaski L., Bartosz G., Zoladz J. A.

Journal of Physiology and Pharmacology

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2010

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tom 61

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nr 6

8

The level of proinflammatory and antiinflammatory cytokines in sperm plasma of fertile and infertile men

80%

Maksymyuk H., Vorobets Z., Maksymyuk V.

Health Problems of Civilization

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2015

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tom 09

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nr 4

9

Endurance training increases plasma brain-derived neurotrophic factor concentration in young healthy men

80%

Zoladz J. A., Pilc A., Majerczak J., Grandys M., Zapart-Bukowska J., Duda K.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 7

10

APO AI-promotor polymorphism influences baseline HDL-cholesterol and apolipoprotein AI concentration in healthy men

80%

Wlodarczyk M., Nowicka G.

Żywienie Człowieka i Metabolizm

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2012

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tom 39

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nr 2

Introduction. Lowlevels of high-density lipoprotein (HDL) cholesterol is a risk factor for coronary artery disease. Besides lifestyle, some gene polymorphisms areknown to be related to the individual HDL-cholesterol concentration. Aim. An apolipoprotein AI gene promoterpolymorphism, related to an adenine (A) to guanine (G) transition 75 base pairs upstream from the transcription initiation site, was studied in the group of healthy men. Methods. Genotyping was performed by polymerase chain reaction method with MspI restriction enzyme. Lipid profile was determined by routine biochemical measurements and apolipoprotein AI concentration was obtain by an immunotubidymetric method. Results. Frequency of the rare A allele was 16% and in the study group only one case of the AA genotype was found, whereas GG variants was observed in 23 participants (68%). Statistically significant differences were noted in HDL concentrations between A allele carriers and GG homozygotes: 56 ± 12 mg/dL vs 46 ± 12 mg/dL, respectively. GG males had also about 20 mg/dL lower apolipoprotein AI levels and about 17 mg/dL higher triglyceride levels. Conclusions. In the studied men of Polish origin the A allele was associated with higher HDL-cholesterol and apolipoprotein AI levels compared to GG homozygotes.

11

80%

Kopjar N., Zeljezic D., Garaj-Vrhovac V.

Acta Biochimica Polonica

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2006

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tom 53

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nr 2

Ograniczanie wyników

Prevalence of EBV genotypes in Polish, Taiwanese and Arabic healthy students and association between genotypes and 30-bp deletion in the LMP-1 gene phylogenetic analysis

Dietary fats and the risk of oxidative stress in a group of apparently healthy women - a short report

The influence of age and gender on the latency of eye movement in healthy humans

Melatonin in human volunteers: focus on nasal lavage level and its free radical scavenging properties with possibility to apply in food research - a short report

Occurrence of Candida spp. in healthy oral microbiota

Assessment of the antioxidant effectiveness of alpha-lipoic acid in healthy men exposed to muscle-damaging exercise

Effect of 5-week moderate intensity endurance training on the oxidative stress, muscle specific uncoupling protein (UCP3) and superoxide dismutase (SOD2) contents in vastus lateralis of young, healthy men

The level of proinflammatory and antiinflammatory cytokines in sperm plasma of fertile and infertile men

Endurance training increases plasma brain-derived neurotrophic factor concentration in young healthy men

APO AI-promotor polymorphism influences baseline HDL-cholesterol and apolipoprotein AI concentration in healthy men

Relative and absolute numbers of T lymphocyte subpopulations and NK cells in male population in relation to age

Impact of classical massage on temperature, strength and flexibility of upper limbs muscles in healthy men

Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: preliminary results

Influence of air pollution on pulmonary function in healthy young men from different regions of Poland

Histologic evaluation of the atrioventricular nodal artery in healthy humans and in patients with conduction disturbances

Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test