Wyniki wyszukiwania

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A novel polymorphism within intron B of growth hormone gene (GH2) of the rainbow trout, Oncorhynchus mykiss

100%

Panicz R., Zych S., Grzesiak W.

Polish Journal of Natural Sciences

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2014

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tom 29

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nr 2

Nucleotide composition of both growth hormone variants of rainbow trout (Oncorhynchus mykiss) has been strongly preserved evolutionally what might suggest that any change within these sequences can have an influence on the functioning of the somatotropic axis. A 121 bp fragment that contained nearly the entire B intron was amplified by the polymerase chain reaction. PCR products were bidirectionally sequenced. PCR products were digested by TaiI according to manufacturer’s instructions and resulting DNA was subjected to electrophoresis. An analysis of the gene fragment for growth hormone 2 showed the presence of SNP, easily identifiable by means of digestion with TaiI restriction enzyme. Statistical analysis confirmed that homozygous GHBB fish were the longest (31.77 cm) and the heaviest (404.70 g) and were statistically significantly different (P ≤ 0.05) from heterozygous GHAB fish. Mean length of GHAA homozygous fish was insignificantly lower (30.06 cm) with mean body weight of 339.12 g than homozygotes GHBB.

2

A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

72%

Qin Y., Tian Y.-P.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 2

The main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/misuse in humans. We determined the global gene expression profile of peripheral whole blood from normal adult male rats after long-term GH gene therapy using CapitalBio 27 K Rat Genome Oligo Arrays. Sixty one genes were found to be differentially expressed in GH gene-treated rats 24 weeks after receiving GH gene therapy, at a two-fold higher or lower level compared to the empty vector group (p < 0.05). These genes were mainly associated with angiogenesis, oncogenesis, apoptosis, immune networks, signaling pathways, general metabolism, type I diabetes mellitus, carbon fixation, cell adhesion molecules, and cytokine-cytokine receptor interaction. The results imply that exogenous GH gene expression in normal subjects is likely to induce cellular changes in the metabolism, signal pathways and immunity. A real-time qRT-PCR analysis of a selection of the genes confirmed the microarray data. Eight differently expressed genes were selected as candidate biomarkers from among these 61 genes. These 8 showed five-fold higher or lower expression levels after the GH gene transduction (p < 0.05). They were then validated in real-time PCR experiments using 15 single-treated blood samples and 10 control blood samples. In summary, we detected the gene expression profiles of rat peripheral whole blood after long-term GH gene therapy and screened eight genes as candidate biomarkers based on the microarray data. This will contribute to an increased mechanistic understanding of the effects of chronic GH gene therapy abuse/misuse in normal subjects.

3

Genetic diversity of Ukrainian Charolais cattle assessed by selected genetic markers

72%

Shkavro N., Radko A., Kozubska-Sobocinska A., Tkachyk T.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

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2016

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tom 34

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nr 2

4

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Growth hormone and growth hormone gene of the American mink (Neovison vison) - the current state of knowledge of one of the key hormones in one of the most intensively economically exploited species

72%

Skorupski J.

Acta Biologica

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2017

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tom 24

5

Transgenic rabbit producing human growth hormone in milk

72%

Lipinski D., Jura J., Kalak R., Plawski A., Kala M., Szalata M., Jarmuz M., Korcz A., Slomska K., Jura J., Gronek P., Smorag Z., Pienkowski M., Slomski R.

Journal of Applied Genetics

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2003

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tom 44

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nr 2

6

Relationship between growth hormone, kappa-casein and beta-lactoglobulin genotypes and selected biochemical blood indicators in young Friesian cattle

72%

Oprzadek J., Lukaszewicz M., Dymnicki E., Zwierzchowski L.

Animal Science Papers and Reports

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2003

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tom 21

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nr 4

Two-hundred-sixty-five young cattle of both sexes, progeny of thirty-one Polish Friesian AI bulls were genotyped for growth hormone (GH), κ-casein (CSN3), and β-lactoglobulin (LGB) gene variants.Associations were evaluated between genetic variants in the three loci and concentration of triiodothyronine (T3), thyroxine (T4), insulin (Ins), glucose (Glu), urea (Ur), creatinine (Cr), cholesterol (Chol), as well as activity of alkaline phosphatase (AP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Only the level of Ins and Ur were not found related to the loci considered. The level of T3 was affected by the interaction between the GH and LGB loci, while that of T4 by the epistasis of GH, CSN3 and LGB. Cr and Chol levels depended on both CSN3 and LGB as well as their interaction. GH × CSN3 loci were found involved in the AP, all the three loci in ALT, and GH × LGB in AST activity moulding.

7

Efektywnosc wzrostu cielat rasy charolaise z uwzglednieniem genetycznego polimorfizmu miostatyny i somatotropiny

58%

Kwasnicki R., Frankowski R.

Acta Scientiarum Polonorum. Medicina Veterinaria

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2007

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tom 06

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nr 2

45-51

8

Zroznicowanie cech jakosci tuszy tucznikow w zaleznosci od polimorfizmu genu hormonow wzrostu [GH-HaeII]

44%

Kmiec M., Kocwin-Podsiadla M., Terman A., Krzecio E., Grzelak T.

Acta Scientiarum Polonorum. Zootechnica

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2010

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tom 09

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nr 2

11-20

Analizowano zależności pomiędzy polimorfizmem GH/HaeII a cechami użytkowości tucznej i rzeźnej tuczników. DNA do badań izolowano z pełnej krwi 369 tuczników należących do pięciu grup rasowych: Landrace, Landrace x Duroc, Landrace x Yorkshire, (Landrace x Yorkshire) x Duroc, (Landrace x Yorkshire ) x (Duroc x Pietrain). Częstość występowania alleli polimorfizmu GH/HaeII wynosiła odpowiednio: A – 0,168 i C – 0,832. Porównując liczebności obserwowane w grupach genotypowych GH/HaeII z liczebnościami teoretycznie skalkulowanymi zgodnie z regułą Hardy’ego-Weinberga nie stwierdzono różnic statystycznie istotnych. Wykazano istotny statystycznie wpływ grupy rasowej na badane cechy użytkowości tucznej i rzeźnej oraz wykazano statystycznie istotną interakcję między grupą rasową a polimorfizmem GH/HaeII dla tempa wzrostu tuczników. Wykazano, że sam polimorfizm GH/HaeII nie różnicował w sposób statystycznie istotny żadnej z analizowanych cech użytkowości tucznej i rzeźnej badanej grupy tuczników.

Ograniczanie wyników

A novel polymorphism within intron B of growth hormone gene (GH2) of the rainbow trout, Oncorhynchus mykiss

A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

Genetic diversity of Ukrainian Charolais cattle assessed by selected genetic markers

Growth hormone and growth hormone gene of the American mink (Neovison vison) - the current state of knowledge of one of the key hormones in one of the most intensively economically exploited species

Transgenic rabbit producing human growth hormone in milk

Relationship between growth hormone, kappa-casein and beta-lactoglobulin genotypes and selected biochemical blood indicators in young Friesian cattle

Efektywnosc wzrostu cielat rasy charolaise z uwzglednieniem genetycznego polimorfizmu miostatyny i somatotropiny

Zroznicowanie cech jakosci tuszy tucznikow w zaleznosci od polimorfizmu genu hormonow wzrostu [GH-HaeII]