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The Grayling Hipparchia semele L. belongs to rapidly vanishing butterfly species in its central and western European range. We studied larval microhabitat preferences of two last viable populations of the Grayling in the Czech Republic. The field study was carried out at two types of human-influenced habitats: a fly ash deposit — a postindustrial site partly left under natural succession processes, and a semi-natural extensively grazed xeric calcareous grassland in May and June 2012 and 2013. During five nights, 89 caterpillars were found (fly ash deposit: 8, xeric grassland: 81). The caterpillars were found on steeper slopes covered with sparse shrubs and shorter herbaceous vegetation with high amount of bare soil or rocks. The most frequently observed behaviour was feeding on fine-leaf fescue grasses with varied size of tufts and rather smaller height up to 20 cm. Usage of larval preferences in a way of active conservation management application is discussed.
Post-industrial sites, including fly ash deposits, are common landscape components in many Central European regions. Their effective restoration is thus crucial because such habitats have been recognised as critical secondary refuges for many endangered and declining species. Controversially, the overwhelming majority of restoration projects consider vegetation units as the restoration target and thus ignore various habitat resources of many endangered species. Our study details habitat-use of the grayling Hipparchia semele, a European endemic xerothermophilous specialist and one of the most rapidly declining butterflies in Central Europe, inhabiting a fly ash deposit in the Kadaň region, western Czech Republic. We estimated its population to 510 males and 346 females by the capture-mark-recapture method during its whole flight period. By detailed recording of all observed specimens' behaviour, we show that this species uses resources from distinct vegetation units, such as exposed and disturbed spots, ruderal regrowths, solitary trees and shrubs, rocks and artificial concrete structures. Because the studied population can act as a source for the whole region, the grayling's ecological needs should be considered in any restoration project. Oppositely, the originally planned restoration of dry grasslands based on plant species composition of vegetation would very probably threaten one of the last two metapopulations in the whole country. Using the grayling's case, we thus warn against the vegetation-based habitat approach in restoration ecology; the resource-based habitat approach should be prioritised, especially when considering needs of the most threatened and/or umbrella species.
Nucleotide sequences of the partial mitochondrial DNA (mtDNA) control region and flanking tRNA Phe gene (a total of 150bp) were determined in eight specimens of European grayling, Thymallus thymallus, representing five rivers in southwestern Poland. Two haplotypes, A and B, were identified which differed from each other by two nucleotide substitutions. Haplotype A was detected in fish from two rivers, the Zadrna and Bóbr, whereas haplotype B was detected in fish from three others, the Widna, Biala Gluchołazka and Metuja. The different phylogenetic position of the mtDNA haplotypes observed in samples from rivers which are in close proximity may suggest genetic structuring of grayling populations in the region, but further sampling is required to test this hypothesis.
In many rheophilic fish species, young stages with low swimming capacities are confined to lateral habitats protected from the main current. Among these lateral habitats, we defined „dead zones” as small bays with shallow water and slow water current caused by physical structures (obstacles or curves of the bank), but without a clear frontier with the main channel. Nevertheless, a study using two and three dimensional hydraulic models revealed the existence of a transiton zone characterized by a strong velocity gradient between these dead zones and the adjacent channel. Young stages of grayling constitute a good model for the study of the different aspects of the use of these lateral habitats. An approach based on direct observation in the field and on experimentations in an artificial channel allowed a precise description of young grayling sizedependent distribution patterns. The grayling undergoes an ontogenetic shift between larval and juvenile habitats. Larval stages are only found in dead zones: the smallest (15-20 mm) are found very close to the river bank and, with increasing age and size (20-40 mm), they begin to get closer to the transition zone. From a size of 40 mm, an increasing number of individuals is observed in the river channel, holding a benthic feeding station. A diel habitat shift also occurs between feeding habitats of larvae and juveniles and dead zones, where the fish are observed resting on the bottom in very shallow water at night. This points out the importance of lateral habitats as (1) exclusive nursery areas for larvae that use them both by day and by night and (2) resting habitats for juveniles at night.
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