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Light and scanning electron microscopy evaluation of the postnatal small intestinal mucosa development in pigs

100%

Skrzypek T., Valverde Piedra J. L., Skrzypek H., Wolinski J., Kazimierczak W., Szymanczyk S., Pawlowska M., Zabielski R.

Journal of Physiology and Pharmacology. Supplement

2005

tom 56

nr 3

71-87

Modifications in the structure of gastrointestinal mucosa is often used to evaluate gut function for instance during the development or in response to particular food components. Scanning electron microscopy (SEM) gives a chance to observe the surface of the gut epithelium in three dimensions. However, this technique is seldom used due to technical difficulties. The present study attempted to investigate the intestinal mucosa structure changes in the postnatal pig using light and scanning electron microscopy technique. Experiments were carried out on sow reared piglets from birth until 38 days of age. Piglets were sacrificed at birth and at the 3rd, 7th, 21st and 38th day of life. The entire gastrointestinal tract was immediately harvested and the whole thickness tissue samples were taken from the duodenum, jejunum and ileum for optical and scanning electron microscopy. SEM analyses corroborated with histometry made by optical microscopy. Moreover, a number of shape modifications of the villi and its surface have been observed. The development changes in small intestine mucosa during the first 3 weeks were manifested in shape, size and density of villi. In conclusion, the structure of small intestinal mucosa undergoes profound structural changes. SEM gives a new dimension in the investigation of gut mucosa.

Preliminary studies on epidermal growth factor [EGF]immunoreactivity in goblet cells of the small intestine by a species-specific antiserum in healthy piglets and piglets with diarrhoea

100%

Claus R., Mentschel J., Blazey B., Munz O.

Journal of Animal and Feed Sciences

2001

tom 10

nr 2

Ograniczanie wyników

1 Journal of Animal and Feed Sciences

1 Journal of Physiology and Pharmacology. Supplement

1 Blazey B.

1 Claus R.

1 Kazimierczak W.

1 Mentschel J.

1 Munz O.

1 Pawlowska M.

1 Skrzypek H.

1 Skrzypek T.

1 Szymanczyk S.

1 Valverde Piedra J. L.

1 Wolinski J.

1 Zabielski R.

1 2005

1 2001

Wyniki wyszukiwania

Light and scanning electron microscopy evaluation of the postnatal small intestinal mucosa development in pigs

Preliminary studies on epidermal growth factor [EGF]immunoreactivity in goblet cells of the small intestine by a species-specific antiserum in healthy piglets and piglets with diarrhoea