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Glucoprotamin antimicrobial activity against selected standard antibiotic-resistant bacteria and reference strains used in the assessment of disinfection efficacy

100%

Chojecka A., Wiercinska O., Rohm-Rodowald E., Kanclerski K., Jakimiak B.

Roczniki Państwowego Zakładu Higieny

2015

tom 66

nr 3

Background. The ability of bacteria to develop common mechanisms of resistance to antibiotics and disinfectants raises doubts about the effectiveness of disinfection processes. Glucoprotamin (GP) is an antimicrobial active substance which is widely used to the disinfection in medical area. Objective. The aim of study was to compare GP’s effectiveness with susceptibility of reference strains used for the evaluation of bactericidal efficacy of disinfectants Staphylococcus aureus (S. aureus); Pseudomonas aeruginosa (P. aeruginosa) and standard antibiotic-resistant strains: meticillin-resistant S. aureus (MRSA) and tetracycline-resistant P. aeruginosa (PAO-LAC). Materials and Methods. Minimum inhibitory concentrations (MICs) of GP and minimum bactericidal concentrations (MBCs) against tested strains were evaluated by serial broth dilution technique. GP’s efficiency was examined according to qualitative (phenol coefficient GP-PC) and quantitative (EN 1040: 2006) test methods. Results. Gram-negative strains were more tolerant to GP than Gram-positive strains among tested strains. MRSA and S. aureus exhibited similar susceptibility to GP. PAO-LAC had significantly lower susceptibility to GP than P. aeruginosa (P≤0,05). There were no differences in GP efficiency against these strains based on GP-PC. According to PN-EN 1040: 2006 standard average obligatory reduction ≥ 5 log10, was demonstrated in the active concentration of GP (84 mg/l) at obligatory 5 min contact time for PAO-LAC and P. aeruginosa. The differences in basis bactericidal activity between PAO-LAC and P. aeruginosa were obtained in the active concentration at 10 and 15 min contact time (P≤0,05). Conclusions. Variation in a susceptibility of reference strains and antibiotic-resistant standard strains has no meaning at used clinically GP concentrations, which are higher than concentration causing basis bactericidal activity of GP.

Antimicrobial activity of glucoprotamin-containing disinfectants

67%

Tyski S., Grzybowska W., Grzeszczuk S., Leszczynski P., Staniszewska M., Rohm-Rodowald E., Jakimiak B.

Polish Journal of Microbiology

2009

tom 58

nr 4

347-353

Introduction of a new antimicrobial agent as a drug - for treatment of infections or as a disinfectant and antiseptic, may result in the occurrence of resistance mechanisms against this agent among microorganisms. Two disinfectants of different composition - Incidin Plus for surface disinfection and Sekusept Plus for medical devices disinfection, both containing glucoprotamin as the active substance, were investigated in this study in order to analyze their antimicrobial activity. Standard bacterial and fungal strains recommended by European Standards, established by European Standardization Committee for testing bactericidal and fungicidal activity of chemical disinfectants were used in the study. Furthermore, 60 clinical bacterial strains with different susceptibility to antibiotics and chemotherapeutics, mostly multiresistant, isolated from different specimens from hospitalized patients were analyzed. In addition, 184 fungal clinical strains isolated from hospitalized patients and outpatients were also included in this study. Antimicrobial activity was evaluated according to EN 1040:2005 - using bacterial strains and according to EN 1275:2005 - using fungal strains. Glucoprotamin proved to be a very effective and rapidly acting bactericidal and fungicidal agent. Low concentration of glucoprotamin - 0.5% showed to be very effective (1 min) against clinical bacterial isolates. Incidin Plus was also very effective (5 min) against clinical fungal isolates.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Roczniki Państwowego Zakładu Higieny

2 Jakimiak B.

2 Rohm-Rodowald E.

1 Chojecka A.

1 Grzeszczuk S.

1 Grzybowska W.

1 Kanclerski K.

1 Leszczynski P.

1 Staniszewska M.

1 Tyski S.

1 Wiercinska O.

1 2015

1 2009

Wyniki wyszukiwania

Glucoprotamin antimicrobial activity against selected standard antibiotic-resistant bacteria and reference strains used in the assessment of disinfection efficacy

Antimicrobial activity of glucoprotamin-containing disinfectants