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1

Molecular mechanism of the apoptotic cell death of glioma cells induced by immunosuppressive drug - cyclosporin A

100%
Kaminska B.
Folia Histochemica et Cytobiologica. Supplement
|
1997
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tom 35
|
nr 2
2
Content available remote

Large-conductance K+ channel openers induce death of human glioma cells

86%
Debska-Vielhaber G., Godlewski M. M., Kicinska A., Skalska J., Kulawiak B., Piwonska M., Zablocki K., Kunz W. S., Szewczyk A., Motyl T.
Journal of Physiology and Pharmacology
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2009
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tom 60
|
nr 4
27-36
Large-conductance Ca2+-activated K+ channels (BKCa channels) are highly expressed in human glioma cells. It has been reported that BKCa channels are present in the inner mitochondrial membrane of the human glioma cell line LN229. In the present study we investigated whether BKCa-channel openers, such as CGS7181 (ethyl 2-hydroxy-1-[[(4-methylphenyl)amino]oxo]-6-trifluoromethyl-1H-indole-3-carboxylate) and CGS7184 (ethyl 1-[[(4-chlorophenyl) amino]oxo]-2-hydroxy-6-trifluoromethyl-1H-indole-3-carboxylate), affect the functioning of LN229 glioma cell mitochondria in situ. In the micromolar concentration range CGS7181 and CGS7184 induced glioma cell death. Morphological and cytometric analyses confirmed that both substances trigger the glioma cell death. This effect was not inhibited by the pan-caspase inhibitor z-VAD-fmk. Lack of DNA laddering, PARP cleavage, and caspase 3 activation suggested that glioma cell death was not of the apoptotic type. We examined the effect of CGS7184 on mitochondrial membrane potential and mitochondrial respiration. Potassium channel opener CGS7184 increased cell respiration and induced mitochondrial membrane depolarization. The latter was dependent on the presence of Ca2+ in the external medium. It was shown that CGS7184 induced an increase of cytosolic Ca2+ concentration due to endoplasmic reticulum store depletion. In conclusion, our results show that CGS7181 and CGS7184 induce glioma cell death by increasing the cytosolic calcium concentration followed by activation of calpains.
3
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Analysis of a fusion between rat glioma cells and biomimetic liposomes with encapsulated diamond nanoparticles or curcumin

72%
Strojny B., Grodzik M., Sawosz E., Gromadka M.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2016
|
tom 55[2]
Analysis of a fusion between rat glioma cells and biomimetic liposomes with encapsulated diamond nanoparticles or curcumin. Liposomes are used as carriers for different bioactive agents, both hydrophilic, which are encapsulated in water core of the liposome, and hydrophobic, which are entrapped within liposome walls. The walls are built from phospholipid bilayer, therefore their structure resembles cell membrane. It was hypothesized that if the wall is made of set of lipids typical for a cell, the liposome will be eagerly consumed by the cell. We performed the experiments using C6 rat glioma cells as an example, since central nervous system cells are extremely rich in lipids, including the unique ones. Since all cancer cells have high proliferation potential, they need to absorb precursors to build cell membrane around new cells, therefore such biomimetic liposomes may be one of the most effective way to deliver anticancer agents into the cell. Analysis of physicochemical properties of obtained liposomes, as well as in vitro tests, showed that obtaining such liposomes is possible and that the liposomes are biocompatible, stable carrier both for hydrophilic and hydrophobic agents. Encapsulation of diamond nanoparticles did not affect the liposomes, whereas entrapping of curcumin, which is a spice known in traditional Asian medicine for its anticancer properties, significantly increased its activity. Obtained results showed that biomimetic liposomes can be effective, individually-tailored carriers for bioactive agents.
4

Regulation of proenkephalin gene expression by transcription factors Fos and CREB: an antisense oligonucleotide approach

58%
Ziolkowska B., Przewlocka B., Bilecki W., Machelska H., Przewlocki R.
Biotechnologia
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1996
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nr 4
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