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1

Comparison of repair activity in different genomic regions

100%
Chakalova L., Russev G.
Acta Biochimica Polonica
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1998
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tom 45
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nr 1
We have developed a quantitative technique to determine repair activity at defined genomic regions. Cells were treated with hydroxyurea to inhibit the replicative DNA synthesis and were incubated with 5-bromodeoxyuridine (BrdUrd) to label the regions undergoing repair. In the course of the labelling, the regions that were more actively repaired would incorporate more BrdUrd than the regions that were less actively repaired. Thus the kinetics of BrdUrd incorporation in the different sequences would reflect the kinetics of reparation of the respective regions. The total BrdUrd-containing, repaired DNA was isolated by immunoprecipitation with anti-BrdUrd antibody, and after controlled sonication, it was used as a template in quantitative PCR in which the amount of the product was directly proportional to the amount of template. This approach was used to address the question whether DNA repair after UV irradiation occurs in an uniformly random manner, or with preferences for certain regions. We found that, in Ehrlich ascites tumor cells, the repair efficiency was higher at the 5' end of the mouse β-globin domain than in the rest of the domain.
2
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A stable density approach to probe selection for a custom aCGH design

84%
Gambin T., Stankiewicz P., Gambin A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2011
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tom 92
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nr 3
3

Genetic associations of reproductive traits in pigs

67%
Kumalska M., Terman A.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
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2017
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tom 56[2]
Genetic associations of reproductive traits in pigs. In the field of genetics, one of the main research area in relation to animal reproduction is the identification of genes or genomic regions influencing reproductive phenotypes. The genes analysed for the determinants of their fertility are among other: LEP, PRL, PRLR, RBP4. With the use of genetic markers, it is possible to identify of both males and females carrying beneficial alleles, and choose reproduce high-quality individuals, which in turn accelerates the genetic improvement of the examined feature. According to literature, about 30% of culling in pig production systems has been primarily due to reproductive problems. Litter size is very important and easily measured reproductive trait, and often included in scientific researches, and defined as the total number of piglets born (TNB) and the number of piglets born alive (NBA). Selection of individuals carrying favourable alleles has the potential to improve reproductive traits and in this connection also sow productive life (SPL). SLP is a measure of the longevity and reproductive performance of a sow and is directly related to the number of viable piglets produced during its lifespan. Because reproductive traits are so multifaceted, researchers are able to consider many different facets of the organism biology to come up with candidate genes and QTLs genes.
4

The Polish Pig Genome Mapping project. XII. Identification of quantitative traits loci afffecting carcass proportions and meat deposition

67%
Pierzchala M., Korwin-Kossakowska A., Cymerowska-Prokopczyk I., Szydlowski M., Kuryl J., Zurkowski M., Kamyczek M., Janik A.
Animal Science Papers and Reports
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2001
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tom 19
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nr 1
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