The level of genetic relatedness of ninety-six strawberry cultivars, released in different breeding centres of seventeen countries, was estimated based on analysis of their DNA polymorphism. Five hundred fifty-eight polymorphic amplicons, with a size range from 80 to 2600 bp, were generated in PCRs carried out on the template of DNA isolated from plants representing all analyzed cultivars. In RAPD reactions, polymorphic bands covered 58% of the total number of PCR products, while in ISSR, SSR and selective AFLP, the polymorphic DNA fragments covered 75%, 70% and 67% of all amplicons, respectively. Data concerning DNA polymorphism were assembled using the PCo-A method (Principal Component Analysis), and then referred to information about country of origin and pedigree described by the breeders. The results showed that contemporary breeding uses genetic resources in a very narrow range. Consequently, the cultivars released in individual breeding centres presented a very close relationship and were grouped in one, or at most two, genetic clusters.
In order to analyze changes in biomass allocation patterns across genetically structured populations where plants are competing for access to light, we performed glasshouse experiment with controlled genetic identity of competitors. Clonal replicates of 17 Lamium maculatum (L.) genotypes were grown in three treatments: control (low competition for light availability), intraclonal and interclonal competition. It was shown that competition between genetically unrelated individuals (interclonal treatment) was the most stressful environment for Spotted Dead Nettle. Results of allometry analyses, based on standardized major axis (SMA) mathematical procedure, have shown the smallest differences in relative investment to sexual reproduction between larger and smaller individuals when competing with unrelated plants. Our results indicate that allometric relationships between vegetative and reproductive traits could be strongly affected by genetic relatedness of competing plants.
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