Wyniki wyszukiwania

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cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI

100%

Zhang J., Dai J., Zhao E., Lin Y., Zeng L., Chen J., Zheng H., Wang Y., Li X., Ying K., Xie Y., Mao Y.

Acta Biochimica Polonica

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2004

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tom 51

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nr 4

Peptidylarginine deiminase (PAD) catalyzes the post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Human, similar to rodents, has four isoforms of PAD (type I, II, III and IV/V), each of which is distinct in substrate specificity and tissue specific expression. In our large-scale sequencing project, we identified a new human PAD cDNA from a human fetal brain cDNA library. The putative protein encoded by this cDNA is designated hPADVI. Expression analysis of hPADVI showed that it is mainly expressed in adult human ovary and peripheral blood leukocytes. We conclude that hPADVI may be orthologous to mouse ePAD, basing on sequence comparison, chromosome localization and exon-intron structure analysis. PAD-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development. This study describes a new important member of the human PAD family.

2

DNA topoisomerase II, intragenic recombination and chromosomal gene organization

86%

Glazkov M. V., Mechtcheriakova J. A.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

3

Nucleotide sequence of nuclear tRNAGly genes and tRNAGly pseudogenes from yellow lupin [Lupinus luteus]: Expression of the tRNAGly genes in vitro and in vivo

72%

Nuc P., Nuc K., Szweykowska-Kulinska Z., Pawelkiewicz J.

Acta Biochimica Polonica

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1997

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tom 44

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nr 2

A nuclear DNA fragment (7.8 kb) from yellow lupin (L. luteus) was sequenced and shown to contain tRNA(Gly) (GGC) genes and tRNAGly (GGC) pseudogenes organized in three tandemly repeated units: of 2565 bp and 2564 bp, and one, truncated from its 3' end, of 1212 bp. Each unit contains an identical pair of a tRNA(Gly) gene and a pseudogene, both having the same polarity. The nucleotide sequence of the gene appears colinear to L. luteus cytoplasmic tRNA(Gly) (GGC) primary structure. All three genes are efficiently transcribed in HeLa-cell nuclear extract giving two primary transcripts. The main, longer primary transcripts have each an extremely long 3' trailer of about 100 nucleotides, the structure of which is specific only for tRNAGly genes and pseudogenes (80% homology) of the studied tandem (but not for other tRNA(Gly) genes of the yellow lupin genome) as it has been shown by Southern hybridization. This distinctive feature allowed to isolate putative tRNAGly precursor(s) encoded by at least one of the three tRNA(Gly) (GGC) genes from L. luteus seedlings.

4

Facilitated hexose diffusion in Kinetoplastida

72%

Szablewski L.

Acta Protozoologica

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2000

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tom 39

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nr 3

5

Cloning of the dam methyltransferase gene from Haemophilus influenzae bacteriophage HP1

58%

Piekarowicz A., Bujnicki J.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 2

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Acta Microbiologica Polonica

1 Acta Protozoologica

1 Cellular and Molecular Biology Letters

1 Bujnicki J.

1 Chen J.

1 Dai J.

1 Glazkov M. V.

1 Li X.

1 Lin Y.

1 Mao Y.

1 Mechtcheriakova J. A.

1 Nuc K.

1 Nuc P.

1 Pawelkiewicz J.

1 Piekarowicz A.

1 Szablewski L.

1 Szweykowska-Kulinska Z.

1 Wang Y.

1 Xie Y.

1 Ying K.

1 Zeng L.

1 Zhang J.

1 Zhao E.

1 Zheng H.

1 2004

2 2000

1 1999

1 1997

cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI

DNA topoisomerase II, intragenic recombination and chromosomal gene organization

Nucleotide sequence of nuclear tRNAGly genes and tRNAGly pseudogenes from yellow lupin [Lupinus luteus]: Expression of the tRNAGly genes in vitro and in vivo

Facilitated hexose diffusion in Kinetoplastida

Cloning of the dam methyltransferase gene from Haemophilus influenzae bacteriophage HP1