Wyniki wyszukiwania

1

Komplexanalyse von Oenothera novae-scotiae Gates, Oe. paradoxa Hudziok und Oe. pseudochicaginesis Rostanski

100%

Sokol S., Rostanski K.

Acta Biologica Silesiana

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1989

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tom 11

2

PLAGL1 protein is differentially expressed in the nephron segments and collecting ducts in human kidney

75%

Godlewski J., Krazinski B. E., Kiezun J., Kwiatkowski P., Sulik M., Tenderenda M., Biernat W., Kmiec Z.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 1

3

Optimization of PCR-RFLP technique for analysis of some genes in the raccoon dog [Nyctereutes procyonoides Gray 1834]

63%

Slaska B., Jezewska G.

Annals of Animal Science

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2008

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tom 08

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nr 1

29-36

4

Analysis of the stress (RYR1) gene and osteochondrosis in wild boars in Lithuania

63%

Klimas R., Klimiene A., Miceikiene I., Morkuniene K.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 12

The aim of the experiment was to determine the RYR1 genotype and to analyse the distribution of osteochondrosis (OC) in wild boars from different regions of Lithuania. Hair and bone samples were collected from wild boars of various sexes and ages that had been shot during hunting season. Genomic DNA was extracted from hair roots. The RYR1 gene was analysed with the PCR-RFLP method, using specific primers and restriction enzymes Alw211 and Hin61. Only one C allele (q = 1.00) and CC genotype of the RYR1 gene were identified. Thus 138 genotyped wild boars were stress-resistant with both dominant alleles intact (no mutation). OC was measured by the cut surface of the distal femur and humerus. The prevalence of OC among the 34 wild boars tested was 20.6%. 5 animals (14.7%) had OC lesions in the hind legs and 2 animals (5.9%) had them in the front legs. OC affected leg joints more often in adult females (third-years and older), but this dependence was not significant (p > 0.05).

5

A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

63%

Qin Y., Tian Y.-P.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 2

The main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/misuse in humans. We determined the global gene expression profile of peripheral whole blood from normal adult male rats after long-term GH gene therapy using CapitalBio 27 K Rat Genome Oligo Arrays. Sixty one genes were found to be differentially expressed in GH gene-treated rats 24 weeks after receiving GH gene therapy, at a two-fold higher or lower level compared to the empty vector group (p < 0.05). These genes were mainly associated with angiogenesis, oncogenesis, apoptosis, immune networks, signaling pathways, general metabolism, type I diabetes mellitus, carbon fixation, cell adhesion molecules, and cytokine-cytokine receptor interaction. The results imply that exogenous GH gene expression in normal subjects is likely to induce cellular changes in the metabolism, signal pathways and immunity. A real-time qRT-PCR analysis of a selection of the genes confirmed the microarray data. Eight differently expressed genes were selected as candidate biomarkers from among these 61 genes. These 8 showed five-fold higher or lower expression levels after the GH gene transduction (p < 0.05). They were then validated in real-time PCR experiments using 15 single-treated blood samples and 10 control blood samples. In summary, we detected the gene expression profiles of rat peripheral whole blood after long-term GH gene therapy and screened eight genes as candidate biomarkers based on the microarray data. This will contribute to an increased mechanistic understanding of the effects of chronic GH gene therapy abuse/misuse in normal subjects.

6

Molecular genetics of Alzheimer's disease: presenilin 1 gene analysis in a cohort of patients from the Poznan Region

51%

Kowalska A., Wender M., Florczak J., Pruchnik-Wolinska D., Modestowicz R., Szczech J., Rossa G., Kozubski W.

Journal of Applied Genetics

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2003

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tom 44

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nr 2

7

Searching for genes controlling fatness traits in pigs - a review

51%

Switonski M., Chmurzynska A., Mackowski M.

Animal Science Papers and Reports

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2003

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tom 21

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nr 2

Fat content of carcass is an important multigenic trait in pig breeding. There are reports indicating several chromosomes, e.g. 1, 2, 4, 5, 6, 7, 12, 13, 14 and 18 which possibly harbour QTLs for fatness traits. Among QTL candidate genes there are leptin (LEP) and its receptor (Lepr), both playing essential role in food intake and energy balance. Moreover, expression level and polymorphism of the adipocyte specific transcription factors, such as CREB (cAMP response element binding protein) or C/EBP (CCAAT/enhancer-binding protein) may also cause phenotypic variation of the fatness traits. Some of the candidate QTLs, as Lep, Lepr, C/EBP and additionally H-FABP (fatty acid binding protein gene) and RYR1, are localized on chromosome 6. It is foreseen that searching for polymorphisms of the chosen genes may reveal association between a genotype and phenotypic variation of selected fatness traits. However, the studies are complex and require analysis of numerous genes.Cited are 71 references.

Ograniczanie wyników

Komplexanalyse von Oenothera novae-scotiae Gates, Oe. paradoxa Hudziok und Oe. pseudochicaginesis Rostanski

PLAGL1 protein is differentially expressed in the nephron segments and collecting ducts in human kidney

Optimization of PCR-RFLP technique for analysis of some genes in the raccoon dog [Nyctereutes procyonoides Gray 1834]

Analysis of the stress (RYR1) gene and osteochondrosis in wild boars in Lithuania

A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy

Molecular genetics of Alzheimer's disease: presenilin 1 gene analysis in a cohort of patients from the Poznan Region

Searching for genes controlling fatness traits in pigs - a review