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Levels of bacterial endotoxin in the samples of settled dust collected in animal houses

100%

Pomorska D., Larsson L., Skorska C., Sitkowska J., Dutkiewicz J.

Bulletin of the Veterinary Institute in Puławy

2009

tom 53

nr 1

37-41

Samples of settled dust were collected in 14 animal houses and examined for the presence of bacterial endotoxin with the Limulus (LAL) test and the gas chromatography - tandem mass spectrometry (GC-MSMS) technique, based on the detection of 3- hydroxy fatty acids (3-OH-FAs) as chemical markers of the endotoxin lipopolysaccharide. The median concentrations of the endotoxin in dust determined with LAL test in sheep sheds, poultry houses, and horse stables were 15,687.5 µg/g, 8,081.8 µg/g, and 79.3 µg/g, respectively, while those determined with the GC-MSMS technique were 868.0 µg/g, 580.0 µg/g, and 496.0 µg/g, respectively. Statistical comparison of the results yielded with LAL test and GC-MSMS technique revealed a weak correlation between both methods. Fatty acids with 14-16 carbon chains (3-OH-C₁₄ and 3-OH-C₁₆) were predominant in the settled dust of the facilities under study. In conclusion, endotoxin in the concentrations detected in this study may present a respiratory hazard to both livestock animals and farm workers. Thus, the prevention measures aiming to lower the exposure to endotoxin in livestock facilities are highly desirable.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Dutkiewicz J.

1 Larsson L.

1 Pomorska D.

1 Sitkowska J.

1 Skorska C.

1 2009

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Levels of bacterial endotoxin in the samples of settled dust collected in animal houses