Fungal mastitis in cows is an increasing issue. The aim of the examination was to evaluate the species and enzymatic activity of yeast-like fungi from mastitis in cows. One hundred and twenty-six strains isolated from secretions of clinically and subclinically inflamed quarters of 102 cows were tested with the use of API 20 C AUX and then 63 strains were examined for enzymatic activity (API ZYM). Candida inconspicua (34.9% ), C. kefyr (19.9% ), C. krusei (11.1%) and S. cerevisiae (10.3%) were the mostly isolated species. Yeast from bovine mastitis showed activity of 12 enzymes, but alkaline phosphatase, valine arylamidase, acid phosphatase and Naphthol-AS-B1-phosphohydrolase were stated at the highest level.
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