Wyniki wyszukiwania

1

Biology and chemistry of fumonisin and AAL toxins

100%

Mirocha C. J., Chen J., Xie W., Xu Y.

Hodowla Roślin, Aklimatyzacja i Nasiennictwo

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1993

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tom 37

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nr 1-2

2

Real-time PCR and agar plating method to predict Fusarium verticillioides and fumonisin B1 content in Nigerian maize

86%

Adejumo T. O., Hettwer U., Nutz S., Karlovsky P.

Journal of Plant Protection Research

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2009

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tom 49

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nr 4

399-404

Eighty maize grain samples collected in Nigeria were investigated for fumonisin B1 (FB1) content and Fusarium verticillioides colonization. F. verticillioides DNA was quantified by species-specific real-time PCR and living propagules of the fungus were counted by agar-plating method. FB1 was detected in 55 (68.7%) of the total samples (mean: 98.5 μg/kg, range: 10 to 714 μg/kg) at 10 μg/kg detection limit. The mean amount of F. verticillioides DNA determined by real-time PCR was 49.7 μg/kg (range: 10-126.7 μg/kg), while agar plate method showed the presence of F. verticillioides in 45 samples (mean incidence: 21.0%, range: 6.7-60.0%). There was correlation ties between F. verticillioides DNA by real time PCR and fungal colonization by agar plate method (R = 0.71, p = 00001 at 95% confidence level), and means of FB1 and F. verticillioides DNA in the yellow and white maize were significantly different. Despite the high consumption of maize in Nigeria, the amount of FB1 ingested by consumers appears to be low. The estimated daily intake of fumonisins was 0.21 μg/kg body weight per day.

3

Mycotoxins biosynthesis by Fusarium oxysporum and F. proliferatum isolates of asparagus origin

72%

Waskiewicz A., Irzykowska L., Karolewski Z., Bocianowski J., Golinski P., Weber Z.

Journal of Plant Protection Research

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2009

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tom 49

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nr 4

369-372

The subject of this work was fumonisin B1 (FB1) and moniliformin (MON) biosynthesis by three isolates of Fusarium oxysporum and three isolates of F. proliferatum of asparagus spears origin. The cultures of fungi were grown on rice and asparagus media for 3 weeks at 20°C. Experiment was carried out in 3 replicates. FB1 and MON occurrence was evaluated with high-performance liquid chromatography (HPLC) analyses. Analysis of variance was carried out to determine biosynthesis of FB1 and MON by F. oxysporum and F. proliferatum. FB1 was found in the amount up to 2012.8 μg/g in cultures of F. proliferatum on rice and in a very small amount in two cultures on asparagus medium. F. oxysporum did not produce FB1 on any of the media. MON was biosynthesized by two the same isolates of F. oxysporum in the amount up to 182.8 μg/g on rice and up to 743.3 μg/g on asparagus medium and one isolate (different on each medium) of F. proliferatum.

4

Occurrence of Fusarium fungi and mycotoxins in marketable Asparagus spears

58%

Waskiewicz A., Irzykowska L., Bocianowski J., Karolewski Z., Kostecki M., Weber Z., Golinski P.

Polish Journal of Environmental Studies

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2010

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tom 19

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nr 1

219-225

Fusarium oxysporum and/or F. proliferatum were isolated from all asparagus spears with brown spots (which indicate an infection) and from almost all spears without spots. The presence of Fusarium spp. And their toxins in the basal parts of asparagus spears was analyzed. Fumonisin B₁ (FB₁) and moniliformin (MON) were found in spears with brown spots and those without disease symptoms. FB₁ was determined in the concentration range 0.16-152.68 ng g⁻¹ (mean 7.52), while moniliformin was detected in the range 15.30-585.00 ng g⁻¹ (mean 121.00). Only in 10% analyzed spears were metabolites not detected.

5

Toxin- and cadmium-induced cell death events in tomato suspension cells resemble features of hypersensitive response

58%

Iakimova E. T., Woltering E. J., Yordanova Z. P.

Journal of Fruit and Ornamental Plant Research

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2007

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tom 15

6

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Cytogenetic effects of Fusarium mycotoxins on root tip cells of rye [Secale cereale L.], wheat [Triticum aestivum L.] and field bean [Vicia faba L.var.minor]

51%

Packa D.

Journal of Applied Genetics

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1997

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tom 38

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nr 3

The effects of fusarial toxins: DAS, T-2, DON, 3 Ac-DON, MON and ZEA, on actively dividing root tip cells of rye, wheat and field bean were investigated. Three concentrations: 1, 5 and 10 µg mL⁻¹ were applied for 24 hours. Nuclei and chromosomes were stained using the Feulgens method. It has been found that trichothecene mycotoxins (DAS, T-2, DON, 3 Ac-DON) had a profound effect on mitosis, as they decreased the mitotic index, produced excessive condensation of pro- and metaphase chromosomes, C-metaphascs and C-anaphases, and caused an accumulation of metaphases. The main effect of trichothecene mycotoxins, probably caused via their influence on protein synthesis, was abnormal functioning of the mitotic spindle.

7

Oznaczanie wybranych mikotoksyn w zywnosci. Cz.II. Dobor optymalnych warunkow oznaczania fumonizyn B1 i B2 w przetworach z kukurydzy metoda wysokosprawnej chromatografii cieczowej

44%

Czerwiecki L.

Roczniki Państwowego Zakładu Higieny

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1998

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tom 49

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nr 1

13-24

Opisano optymalne warunki oznaczania fumonizyn B1 і В2 w przetworach z kukurydzy. Zbadano przebieg procesu ekstrakcji, oczyszczania ekstraktów, tworzenia fluoryzujących pochodnych fumonizyn oraz wysokosprawnej chromatografii cieczowej. Średni odzysk metody w zależności od poziomu fortyfikacji oraz rodzaju badanej próbki wynosił 64-95%, granica wykrywalności 15 µg każdej z fumonizyn w kilogramie produktu.

Ograniczanie wyników

Biology and chemistry of fumonisin and AAL toxins

Real-time PCR and agar plating method to predict Fusarium verticillioides and fumonisin B1 content in Nigerian maize

Mycotoxins biosynthesis by Fusarium oxysporum and F. proliferatum isolates of asparagus origin

Occurrence of Fusarium fungi and mycotoxins in marketable Asparagus spears

Toxin- and cadmium-induced cell death events in tomato suspension cells resemble features of hypersensitive response

Cytogenetic effects of Fusarium mycotoxins on root tip cells of rye [Secale cereale L.], wheat [Triticum aestivum L.] and field bean [Vicia faba L.var.minor]

Oznaczanie wybranych mikotoksyn w zywnosci. Cz.II. Dobor optymalnych warunkow oznaczania fumonizyn B1 i B2 w przetworach z kukurydzy metoda wysokosprawnej chromatografii cieczowej