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1

Purification and characterization of an antibacterial protein from dried fruiting bodies of the wild mushroom Clitocybe synopica

100%
Zheng S., Liu Q., Zhang G., Wang H., Ng T. B.
Acta Biochimica Polonica
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2010
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tom 57
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nr 1
43-48
 A novel antibacterial protein with a molecular mass of 44 kDa has been isolated from dried fruiting bodies of the wild mushroom Clitocybe sinopica. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis showed that the protein was composed of two subunits each with a molecular mass of 22 kDa. Its N-terminal amino-acid sequence, SVQATVNGDKML, has not been reported for other antimicrobial proteins. The purification protocol included ion exchange chromatography on DEAE-cellulose, CM-cellulose and Q-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The antibacterial protein was adsorbed on all three ion exchangers. The antimicrobial activity profile of the protein against tested bacterial and fungal strains disclosed that it possessed potent antibacterial activity against Agrobacterium rhizogenes, A. tumefaciens, A. vitis, Xanthomonas oryzae and X. malvacearum with a minimum inhibitory concentration mostly below 0.6 μM. However, it had no antibacterial activity against Pseudomonas batatae, Erwinia herbicola, Escherichia coli, and Staphylococcus aureus, and no antifungal activity against Setosphaeria turcica, Fusarium oxysporum, Verticillium dahliae, Bipolaris maydis, and B. sativum. The antibacterial antivity against A. tumefaciens was stable after exposure to 20-60°C for 30 min and to pH 4-9 for 1 h.
2
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Extraction of cordycepic acid from the fruiting body of Cordyceps militaris (L.)

100%
Do T. H., Nguyen H. C.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2019
|
tom 100
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nr 3
3
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Impact of freeze drying on the contents of biologically active substances in the fruiting bodies of selected edible mushroom species

100%
Kalbarczyk J.
Acta Agrophysica
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2003
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tom 02
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nr 2[96]
4

A novel alkaline protease with antiproliferative activity from fresh fruiting bodies of the toxic wild mushroom Amanita farinosa

84%
Sun J., Zhao Y., Chai H., Wang H., Ng T. B.
Acta Biochimica Polonica
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2011
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tom 58
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nr 4
A novel protease with a molecular mass of 15 kDa was purified from fresh fruiting bodies of the wild mushroom Amanita farinosa. The purification protocol entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The protease was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and SP-Sepharose. It demonstrated a single 15-kDa band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE) and a 15-kDa peak in gel filtration. The optimal pH and optimal temperature of the protease were pH 8.0 and 65 °C, respectively. Proliferation of human hepatoma HepG2 cells was inhibited by the protease with an IC50 of 25 µM. The protease did not have antifungal or ribonuclease activity.
5
Content available remote

Popular species of edible mushrooms as a good source of zinc to be released to artificial digestive juices

84%
Zajac M., Muszynska B., Kala K., Sikora A., Opoka W.
Journal of Physiology and Pharmacology
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2015
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tom 66
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nr 5
6

Activity of Cs-137 radionuclides in fruiting bodies of selected mushrooms species growing in Poland

84%
Grzybek J., Jasinska M., Kozak K., Mietelski J.
Acta Poloniae Toxicologica
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1996
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tom 04
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nr 2
7
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Analysis of indole compounds in fruiting bodies and in mycelia from in vitro cultures of Calocera viscosa (Basidiomycota)

67%
Muszynska B., Sulkowska-Ziaja K.
Acta Mycologica
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2012
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tom 47
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nr 1
Calocera viscosa (Pers.: Fr.) Fr. (Basidiomycota) from Dacrymycetaceae family is a widespread species of mushroom in Poland. The aim of this study was to investigate the content of indole compounds in fruiting bodies and in mycelium cultured in vitro on solid and liquid medium of this species. Fruiting bodies of Calocera viscosa were collected in coniferous forests in south Poland and were used to derive in vitro cultures. The optimal medium composition for cultures was determined. Fresh material: fruiting bodies and mycelium from culture in vitro was frozen and then dried by lyophilization. The crushed dry biomass was extracted with petroleum ether to remove oil fraction, material was dried and extracted with methanol. Analysis of indole compounds was performed in methanol extracts using chromatographic methods: TLC, UV Vis, EIMS and HPLC. This analysis presented in all three extracts the following indole compounds: L-tryptophan, 5-hydroxytryptophan, 5-methyltryptophan, melatonin and indole (contents fluctuated in the range: 0.37 to 11.88 mg/100 g d.w.). 5-hydroxytryptophan contents in all extracts were significant and amounted to 11.88 mg/100 g d.w. in fruiting bodies, and 11.42 in mycelium from liquid cultures and and 10.59 in mycelium from solid cultures. In addition, the fruiting bodies and mycelium from cultures on liquid medium revealed the presence of serotonin (0.39 and 3.19 mg/100 g d.w. respectively).
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