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Ejaculates from three rams were collected with artificial vagina at one day (OD1) and four day intervals (FDsI). Part of the semen samples were pooled, diluted with skim milk based extender: -egg yolk (10%) -glycerol (5%), loaded into 0.25 ml straws, equilibrated, frozen, and afterwards stored in liquid nitrogen. The following spermatozoon parameters were determined in fresh and frozen-thawed semen: motility, viability, abnormality, abnormal acrosome, and membrane integrity. Semen collected at ODI had significantly higher freeze-ability compared with semen collected at FDsI for spermatozoon motility. Frozen-thawed semen collected at ODI had higher viability and membrane integrity, and lower abnormality and abnormal acrosome percentages compared with FDsI. It was concluded that in order to use cryopreservation of the Tushin ram semen, ODI instead of FDsI collection should be preferred.
A limited field trial was performed to evaluate the fertilizing capacity of boar spermatozoa frozen in an extender supplemented with lipoprotein fractions isolated from ostrich egg yolk (LPFo). Boar semen, diluted in an extender containing lactose with lyophilized lipoprotein fractions, glycerol and Orvus Es Paste (lactose-LPFo-G), was frozen using a controlled programmable freezer. Sperm characteristics, such as motility, plasma membrane and acrosome integrity, and mitochondrial function were monitored. Post-cervical artificial inseminations (post-CAIs) in multiparous sows (Polish Large White) were performed using the Soft & Quick® catheter/cannula set. Sows were inseminated 2 to 3 times within one oestrus. Possible returns of sows to oestrus were determined from 21 to 30 days after post-CAIs. In this field trial, sows inseminated with 2 x 109 motile frozen-thawed spermatozoa resulted in pregnancy and farrowing rates of 75%, respectively. The average piglets born live was 10.5 ± 0.4 (mean ± SEM). The data of this study showed that post-CAI of boar semen frozen in LPFo-containing extender has the potential to provide acceptable fertility results. Further investigations are needed to elucidate the cause of variations in pregnancy/farrowing rate associated with frozen-thawed boar semen.
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