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1

Involvement of Naplus-Hplus exchanger in desmopressin-induced platelet procoagulant response

100%
Tomasiak M. M., Stelmach H., Bodzenta-Lukaszyk A., Tomasiak M.
Acta Biochimica Polonica
|
2004
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tom 51
|
nr 3
Desmopressin (DDAVP) action on platelets is associated with the development of procoagulant response but the underlying mechanism of this phenomenon is not known. We investigated whether this effect of DDAVP might be due to activation of plasma membrane Na+ /H+ exchanger. The DDAVP-induced platelet procoagulant re­sponse, measured as phospholipid-dependent thrombin generation, was dose de­pendent and significantly weaker than that produced by collagen or monensin (mim­ics Na+ /H+ antiport). Both the DDAVP- and collagen-produced procoagulant re­sponses were less pronounced in the presence of EIPA, an Na+/H+ exchanger inhibi­tor. Flow cytometry studies revealed that in vitro treatment of platelets with DDAVP or collagen was associated with the appearance of both degranulated (and frag­mented) and swollen cells. The DDAVP-evoked rise in size and granularity heteroge­neity was similar to that produced by collagen or monensin and was not observed in the presence of EIPA. Using flow cytometry and annexin V-FITC as a probe for phosphatidylserine (PS) we demonstrated increased and uniform binding of this marker to all subsets of DDAVP-treated platelet population. The DDAVP-evoked PS expression was dose dependent, strongly reduced by EIPA and weaker than that caused by monensin or collagen. As judged by optical swelling assay, DDAVP in a dose dependent manner produced a rise in platelet volume. The swelling was inhib­ited by EIPA and its kinetics was similar to that observed in the presence of monensin. Electronic cell-sizing measurements showed an increase in mean platelet volume and a decrease in platelet count and platelet crit upon treatment with DDAVP. DDAVP elicited a slow (much slower than collagen) alkalinization of platelet cytosol. Altogether the data indicate an involvement of Na+/H+ exchanger in the generation of procoagulant activity in DDAVP-treated platelets.
2

The patterns of MHC association in aplastic and non-aplastic paroxysmal nocturnal hemoglobinuria

84%
Nowak J., Mika-Witkowska R., Mendek-Czajkowska E., Rogatko-Koros M., Graczyk-Pol E., Pyl H., Klimczak A., Wojcik M., Prochorec-Sobieszek M., Maryniak R., Zupanska B.
Archivum Immunologiae et Therapiae Experimentalis
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2011
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tom 59
|
nr 3
3

Partial reversal of transformed fusiform phenotype by overexpression of calreticulin

84%
Opas M., Fadel M. P.
Cellular and Molecular Biology Letters
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2007
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tom 12
|
nr 2
Calreticulin, a Ca2+-storage and chaperone protein of the ER, has also been shown to affect cell adhesiveness. To examine the effects of differential expression of calreticulin on cellular adhesiveness, we used L fibroblast cell lines stably expressing either elevated or reduced amounts of full length, ER-targeted calreticulin. Overexpression of calreticulin correlates with an increase in adhesiveness of L fibroblasts such that these transformed cells acquire epithelioid morphology and form an epithelial-cell sheet when crowded. Functionally, the “reversal” of transformed phenotype in L fibroblasts differentially overexpressing calreticulin can be accounted for by changes in levels of expression of N-cadherin and vinculin. Structurally, however, although the form and extent of cell-cell contacts in L fibroblasts overexpressing calreticulin mimicked those in normal epithelia, electron microscopical examination revealed that cell-cell junctions formed by these transformed cells bore only superficial resemblance to those of normal epithelia in culture. Our data imply that overexpression of calreticulin, while partially reverses fusiform transformed phenotype is in itself insufficient to re-establish bona fide zonulae adherens in transformed fibroblasts.
4

Prevalence of antibodies to Toxoplasma gondii and Neospora caninum in wildlife and farmed foxes (Vulpes Vulpes)

67%
Smielewska-Los E., Pacon J., Janczak M., Ploneczka K.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
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2003
|
tom 06
|
nr 2
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