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The effect of florfenicol on lymphocyte subsets and humoral immune response in mice

100%
Lis M., Szczypka M., Suszko A., Switala M., Obminska-Mrukowicz B.
Polish Journal of Veterinary Sciences
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2011
|
tom 14
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nr 2
Florfenicol is a broad-spectrum bacteriostatic antibiotic used in domestic animals. The aim of the study was to determine the effect of florfenicol on the total number of lymphocytes in the thymus, spleen and mesenteric lymph nodes and the percentage and the absolute number of T cell subsets (CD4+CD8+, CD4-CD8-, CD4+, CD8+) in the thymus and T (CD3+, CD4+, CD8+) and B (CD19+) lymphocytes in the peripheral lymphatic organs in non-immunized mice and humoral immune response in sheep red blood cells (SRBC)-immunized mice. Florfenicol was administered orally at a dose of 30 mg/kg six times at 24 h intervals to non-immunized mice and four or seven times at 24 h intervals to SRBC-immunized mice. SRBC was injected 2 hours prior to the first dose of the drug. Florfenicol increased the percentage of CD4-CD8- thymocytes and the absolute number of CD4+ and CD8+ thymocytes on day 7. The increased percentage and absolute number of CD3+, CD4+ and CD8+ lymphocytes in mesenteric lymph nodes and decreased percentage of lymphocytes B were also observed 24 hours from the last administration of florfenicol. Florfenicol administered after SRBC immunization reduced the number of plaque forming cells (PFC) and the production of anti-SRBC antibodies on days 4 and 7 after priming.
2

Efficacy of florfenicol and flunixin followed with vitamins E and/or C on selected oxidative and inflammatory mechanisms in young cattle under transport and adaptation stress

80%
Wernicki A., Stachura R., Hola P., Puchalski A., Dec M., Stegierska D., Zurek A., Urban-Chmiel R.
Medycyna Weterynaryjna
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2018
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tom 74
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nr 04
Bovine respiratory disease (BRD) in cattle, due to the participation of numerous viral and bacterial etiological agents, predisposition factors, and environmental stresses, is a serious health and economic problem in herds of beef cattle. Given the broad scope of the problem of respiratory syndrome and the limited immunoprophylaxis, the aim of this study was to estimate the additive effects of flunixin with florfenicol followed with vitamin E or/and C in reducing the development of oxidative stress and inflammation in the first weeks in the feedlot. The study was conducted on young Simmental cattle (n = 50) weighing approx. 160 kg. The animals were divided into 5 groups (n = 10 in each group). Group I was the control. The cattle received florfenicol and flunixin in combination with vitamins E or C (Group II and III), florfenicol and flunixin followed with vitamin E and C (Group IV), or florfenicol and flunixin without vitamins (group V). Blood for sera was collected on the day the drugs were administered and on days 3, 7, 14, 21 and 28 of the experiment. The level of oxidative stress was analysed on the basis of the concentration of nitrogen ions (NO) in the sera and of lipid peroxidation end products reacting with thiobarbituric acid (TBARS). Concentrations of haptoglobin (Hp) and serum amyloid A (SAA) in the sera were determined using EIA enzyme immunoassays. The treatment used in the study, involving the application of florfenicol and flunixin with vitamin E or C, substantially reduced the level of oxidative stress, expressed as a reduced concentration of TBARS and NO ions, particularly in groups II and III. It was also confirmed that the treatment inhibited the concentration of acute phase proteins (Hp and SAA).
3

In vitro study on the effect of chloramphenicol and florfenicol on cellular and humoral immunity of carp [Cyprinus carpio] and rabbit [Oryctolagus cuniculus]

80%
Sieroslawska A., Studnicka M., Siwicki A. K., Bownik A., Rymuszka A.
Acta Poloniae Toxicologica
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2000
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tom 08
|
nr 1
4

Leczenie zapalenia uszu u psów. Tak wiele możliwości - którą wybrać?

51%
Cole L.
Weterynaria po Dyplomie
|
2019
|
tom 20
|
nr 5
5

Wykrywanie i oznaczanie pozostalosci florfenikolu i tiamfenikolu w tkankach jadalnych zwierzat metoda elektroforezy kapilarnej

51%
Kowalski P., Plenis A., Oledzka I.
Bromatologia i Chemia Toksykologiczna
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2009
|
tom 42
|
nr 3
569-573
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