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In Poland Galanthus nivalis L. is partially protected. The flowers of this species are one of the first sources of nectar and pollen for insects from February to April. The aim of this study was to present the flowering biology as well as the topography, anatomical, and ultrastructural features of the floral nectary. The flower lifespan, the breeding system, and the mass of pollen and nectar produced by the flowers were determined. Examination of the nectary structure was performed using light, fluorescence, scanning and transmission electron microscopy. The flower of G. nivalis lives for about 30 days. The stamens and pistils mature simultaneously and during this time nectar is secreted. The anthers of one flower produced the large amount of pollen (4 mg). The breeding system of G. nivalis was found to be characterized by partial self-compatibility, outcrossing, and xenogamy. The nectary is located at the top of the inferior ovary. The nectary epidermal cells are characterized by striated cuticular ornamentation. Initially, the secreted nectar formed vesicle-like protuberances under the cuticle. The epidermal and parenchymal cells contain numerous plastids, mitochondria, dictyosomes, ER cisterns, and vesicles fused with the plasmalemma, which indicates granulocrine nectar secretion.
The conducted study related to the structure of the floral nectaries of Rhododendron japonicum (A. Gray) J. V. Suringar ex E. H. Wilson. The structure of the secretory epidermis of the nectaries was analysed by using scanning electron microscopy (SEM). Rhododendron japonicum develops the superior pistil with a 5-loculed ovary equipped in five ribs. The nectary gland is located in the lower part of the ovary. In the nectary regions located on the extension of the ribs of the ovary, stomata were very numerous. In the upper part of the nectary, stomata were arranged individually or in small clusters, whereas at its half-height they formed stomatal areas. The stomata were at different growth stages. They were arranged in different directions. The stomata developed on the nectary surface according to the mosaic pattern. The stomata from the lower situated part of the nectary had a different structure than those occurring in the upper half of the nectary. The stomata in the nectaries of Rh. japonicum belong to the actinocytic type. The cuticle layer in the upper part of the nectary was better developed and had a characteristic sculpture, whereas in the lower part it was smooth.
The structure of receptacular surfaces of floral nectaries at two flowering stages and the structure of the outer surface of the receptacle of Sorbus aucuparia were investigated using scanning electron microscopy. Changes in the development of the cuticular epithelium of the nectary epidermis and differences in the degree of aperture of stomata were observed. Increased undulation of the gland surface was found during flower development. Numerous stomata were situated slightly below the level of epidermal cells of the nectary. At the pollination stage, open pores or pores surrounded by the cuticular epithelium were observed, as well as covered by dried secretion. Dried nectar in the form of patches was also visible on the surface of the gland. Stomata of the outer surface of the receptacle were located on protrusions and surrounded by the cuticular epithelium.
The study of floral nectary structures of thirteen ornamental apple cultivars examined using light microscope (MS) and scanning electron microscope (SEM) was performed. It was found that nectary glands in the selected cultivars were located in the upper part of the flower receptacle, between the ovary of the pistil and the base of stamen filaments, and they generally belonged to the epimorphic or transitoric type. The nectary surface area, its thickness, the number of glandular tissue layers, the height of epidermal cells of the nectary and the thickness of the outer wall of the epidermis, together with the cuticle, were determined by light microscope. By using SEM, the structure of the surface of nectaries in four ornamental apple cultivars was observed. The epidermis of the upper part of the nectaries was composed of elongated cells of which outer cell wall was covered with a striated cuticle. The remaining part of the nectary was characterised by cells of similar arrangement and shape, but their surface was marked by a thinner and smoother layer of cuticle. Closed or opened stomata were generally situated at the level of the epidermal cells. Their pores were often filled with granular or plate-shaped structures.
The structure of septal nectaries in Asphodelus aestivus flowers was investigated by using light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (EM). It was found that the outlets of the three parts of the nectary were situated on the ovary surface at 2/3 of its height and had the shape of elongated openings. The nectariferous tissue was in the septa of the lower part of the ovary. The secretory tissue cells formed 1-3 layers surrounding the nectary slits. They contained thin cell walls with the cuticle layer from the slit side, large cell nuclei, numerous mitochondria and plastids characterised by various shapes. In plastids, small starch grains occurred sporadically. At the beginning of anthesis, the cells were poorly vacuolized. ER cisternae and secretory vesicles were located near the outer cell wall. Fibrous substance was present in the nectary slits. In the subglandular tissue, numerous starch grains occurred at the beginning of anthesis. In this zone, cells containing raphides and xylem elements were observed. Based on the ultrastructure of the nectary it can be stated that granulocrine nectar secretion occurs in A. aestivus.
Badano cechy morfologiczne i anatomiczne oraz związane z ultrastrukturą komórek nektarników Achillea millefolium z rodziny Asteraceae. Obecność nektarników stwierdzono tylko w kwiatach rurkowatych u podstawy szyjki słupka. Zbadano mikromorfologię nektarników w SEM, przeprowadzono obserwacje struktury w mikroskopie świetlnym i transmisyjnym elektronowym. Określono liczbę warstw budujących gruczoł, wielkość oraz kształt komórek epidermy i komórek gruczołowych. Analizowano ultrastrukturę komórek wydzielniczych. Dyskoidalny gruczoł nektarnikowy oglądany z góry miał kształt pięciokąta o wysokości 181,5 µm i średnicy 299,4 µm. Zbudowany był z jednowarstwowej epidermy oraz średnio z 6 warstw komórek wydzielniczych. Komórki gruczołowe były większe (27 µm) od komórek skórki (22 µm), różny był także kształt komórek w obu tkankach. Sekrecja nektaru odbywała się przez zmodyfikowane aparaty szparkowe. Komórki aparatów szparkowych były wyniesione ponad powierzchnię pozostałych komórek tkanki okrywającej oraz wyróżniały się większymi rozmiarami. Nektarniki zaopatrywane były przez wiązki waskularne biegnące od szyjki słupka, które kończyły się u podstawy nektarnika, nie wnikając w głąb gruczołu. Badane w TEM komórki epidermy nektarnika krwawnika charakteryzowały się obecnością dużych jąder komórkowych, licznych piastydów, mitochondriów oraz wakuol z depozytami włóknistej wydzieliny oraz pęcherzykowatymi strukturami. W komórkach tkanki wydzielniczej nektarnika obserwowano elektronowo gęstą cytoplazmę, liczne plastydy, mitochondria, aparaty Golgiego oraz rozległą sieć retikulum endoplazmatycznego.
The performed studies focused on the surface structure of floral nectaries of four species from the following genus: C. coccinea L , C. crus-galli L, C. curvisepala Lindm and C. prunifolia (Poiret) Pers. The observations of the epidermis area were made in a scanning electron microscope (SEM). A nectary appears to be shaped like a slightly curved disk situated between the pistil style and the basal part of the stamens filaments. The nectary area of the studied species differed substantially as regards the cuticle sculpture and stomata number. The nectary secretion in Crataegus flowers proceeds through the stomata located below a level of the other epidermis cells, in the deep indents of the secretory tissue. The highest stomata number in 1 mm² nectary epidermis was recorded in C. crus-galli, C. coccínea, C. prunifolia and finally, C. curvisepala. Analyzing the nectary cuticular structure in respect of its increasing complexity (absence or presence of stripes), the investigated taxons can be ordered as following: Crataegus curvisepala, C. coccinea, C. crus-galli and C. prunifolia.
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