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Background. Commonly occurring diseases can have the origin in oxidative processes ongoing in the human body. Vegetables of Brassicaceae family are the essential sources of natural antioxidants, especially phenolic compounds, in the human diet. The research was aimed to estimate the content of phenolic compounds in selected vegetables and their quantity changes during hydrothermal processes. Material and methods. The vegetables subjected to analysis were: kale, broccoli, Brussels sprouts, and white and green cauliflower. The fresh and processed (blanched, cooked, frozen, cooked after freezing) vegetables were freeze-dried. The levels of phenolic acids and flavonols by HPLC method were estimated. Results. The presence of derivatives of hydroxycinnamic acid, mainly of caffeic acid, p-coumaric acid, sinapic acid and of flavonols - kaempferol, and in smaller amounts of quercetin was found. The largest amounts of above components were present in kale (total 94.4 mg-100 g'1 of fresh matter), whereas the smallest amounts were found in white and green cauliflower - 3.6 mg-100 g’1 f.m. and 3.03 mg-100 g'1 f.m., respectively. The applied technological processes contributed to lover amounts of all tested compounds depending on the process and the vegetable kind. The biggest loses, up to 70-80%, took place during cooking of raw and previously frozen vegetables. Conclusions. Analysed Brassicaceae were characterized by high contents of the investigated flavonoids. The best source of those compounds was kale whereas the smallest amounts of searched components were presented in cauliflowers. The used hydrothermal processes led to losses of searched compounds.
This study examined the effects of cytokinins and methyl jasmonate (JAMe) alone and in combination on the growth and flavonol content of zucchini cotyledons cultured in the presence or absence of Cu2+. During greening of cotyledons in intensive diurnal illumination, rutin was identified as the main flavonol compound. Its accumulation was greatly stimulated by phenylurea cytokinin (4PU-30) but reduced by Cu2+. Stimulation by benzylaminopurine (BA) was less. JAMe showed an inhibitory effect, alone and with simultaneous addition of Cu2+ or cytokinins. In moderate excess (100 μM and 250 μM CuSO4), Cu2+ enhanced stimulation by 4PU-30 of rutin accumulation; at a higher concentration or with other substances it decreased the rutin level. The other flavonol detected, kaempferol-3-rhamnoside, increased after JAMe or 4PU-30 were added; in the other cases it decreased. The data obtained indicate that cytokinins can increase rutin content in developing Cucurbita cotyledons. Rutin content usually decreased under stress induced by excess Cu2+, but Cu2+ in moderate excess had a stimulating effect in the presence of higher phenylurea cytokinin levels
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