The aim of this examination was optimization of flavonols' detection method in plant's extracts. The leaves of Rosa canina L. and Rosa damascena Mill, were chosen as the experimental material, due to their rich content of flavonols. In the study, optimization and comparison of extraction, hydrolysis and purification processes of samples predestinated to HPLC analysis of flavonol aglycones were performed. The most optimal method to detect flavonols in examined material was their direct hydrolysis with 1.2 N HCL during 1 h connected with its extraction with 50% methanol and then purification of the sample by SPE before HPLC examination. The comparison and evaluation of two elaborated methods of hydrolyzate purification from neutral compounds, which are liquid-liquid extraction (LLE) with ethyl acetate and solid phase extraction (SPE) in RP-18 microcolumns, were carried out. 78% recovery of standard quercetin during LLE and 98.8% recovery of standard quercetin during filtering and elution of hydrolyzate with methanol through octadecyl microcolumns in SPE method were observed. These results showed that SPE is better then LLE during superior sample purification procedure.
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