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Isolation and characterization of caprine arthritis encephalitis virus in goats from Poland

100%

Kaba J., Rola M., Materniak M., Kuzmak J., Nowicki M.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 2

183-188

The caprine arthritis-encephalitis virus (CAEV) was isolated from monocyte-derived macrophages (M/M), but not from PBMC of seropositive goats by co-cultivation with goat synovial membrane cells. Out of eight M/M co-cultures, CAEV was evidenced by the syncytia formation and presence of proviral DNA in two and four cultures, respectively. Two virus isolates from co-cultures showing cytopathic effects were further confirmed as CAEV by western blotting, PCR, and sequence analysis. The nucleotide sequence of gag gene showed 92.0% and 90.3% homology to the prototype CAEV-Co strain. Supernatants harvested from these cultures induced syncytia when cultured with uninfected cells and the resultant titer was 1035 and 1025 TCID50 per ml. New CAEV isolates are suitable candidates for further analysis of their genetic and biological properties.

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Isolation and partial characterization of bovine foamy virus from Polish cattle

84%

Materniak M., Bicka L., Kuzmak J.

Polish Journal of Veterinary Sciences

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2006

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tom 09

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nr 4

The first isolation and partial characterization of bovine foamy virus (BFV), also known as bovine syncytial virus, in Poland is described. This virus was isolated by co-cultivation of peripheral blood leukocytes from infected cattle with permissive Cf2Th cells. The new isolate, called BFV100 was identified using several techniques: electron microscopy, western blotting, PCR and sequencing of a part of the gag and pol/env genes. Based on syncytia induction, antigenic determinants, primer binding sites and sequence analysis, it can be concluded that isolate BFV100 is bovine foamy virus and is related to the known American and German BFV isolates by sequence homology and antigenic relatedness.

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Tolerance of Venturia inaequalis spp. to ergosterol biosynthesis inhibitor fungicides in Poland. Part II. The differences in sensitivity of field isolates of Venturia inaequalis [Cooke] Aderh. to ergosterol biosynthesis inhibitors fungicides

84%

Nowacka H.

Journal of Fruit and Ornamental Plant Research

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1995

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tom 03

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nr 2

Ograniczanie wyników

2 Polish Journal of Veterinary Sciences

1 Journal of Fruit and Ornamental Plant Research

2 Kuzmak J.

2 Materniak M.

1 Bicka L.

1 Kaba J.

1 Nowacka H.

1 Nowicki M.

1 Rola M.

1 2009

1 2006

1 1995

Isolation and characterization of caprine arthritis encephalitis virus in goats from Poland

Isolation and partial characterization of bovine foamy virus from Polish cattle

Tolerance of Venturia inaequalis spp. to ergosterol biosynthesis inhibitor fungicides in Poland. Part II. The differences in sensitivity of field isolates of Venturia inaequalis [Cooke] Aderh. to ergosterol biosynthesis inhibitors fungicides