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1

Is sex worth searching for?

100%
Witt M.
Archivum Immunologiae et Therapiae Experimentalis
|
1994
|
tom 42
|
nr 5-6
2

Fetal-cell microchimerism, lymphopoiesis, and autoimmunity

67%
Leduc M., Aractingi S., Khosrotehrani K.
Archivum Immunologiae et Therapiae Experimentalis
|
2009
|
tom 57
|
nr 5
325-329
3
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Pseudophysiological transcomplementary activation of reconstructed oocytes as a highly efficient method used for producing nuclear-transferred pig embryos originating from transgenic foetal fibroblast cells

67%
Samiec M., Skrzyszowska M., Lipinski D.
Polish Journal of Veterinary Sciences
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2012
|
tom 15
|
nr 3
The completely new strategy of pseudophysiological transcomplementary (transcytoplasmic) activation (PP-TCA) of nuclear-transferred oocytes, which had been derived from pWAPhGH-GFPBsd transfected foetal fibroblast cells, was recently applied to the somatic cell cloning of pigs. It resulted in the considerable enhancing not only the cleavage activity of cultured cloned embryos, but also their morula and blastocyst formation rates as compared to the use of standard simultaneous fusion and electrical activation of reconstituted oocytes (77% vs. 57%, 63% vs. 46% and 40% vs. 27%, respectively). Altogether, the use of cytosolic components descended from heterologous (rabbit) zygotes as the agents for stimulation of porcine clonal cytoplasmic hybrids (cybrids) turned out to be reliable and feasible strategy for the generation of transgenic blastocysts by somatic cell nuclear transfer (SCNT). Furthermore, to our knowledge, no previous study has reported the preimplantation developmental outcome of transgenic nuclear-transferred pig embryos following the PP-TCA that was developed and optimised in our laboratory.
4

Prenatal development of the adrenal gland in the pig (Sus scrofa domestica). Part I. Differentiation and development of the adrenal cortex primordium in the first half of pregnancy

67%
Bielanska-Osuchowska Z.
Folia Morphologica
|
1989
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tom 48
|
nr 1-4
5

Engrafting fetal liver cells into multiple tissues of healthy adult mice without the use of immunosuppressants

67%
Darinskas A., Gasparaviciute R., Malisauskas M., Wilhelm K., Kozhevnikov J. A., Liutkevicius E., Pilinkiene A., Morozova-Roche L. A.
Cellular and Molecular Biology Letters
|
2007
|
tom 12
|
nr 3
We have shown the fetal liver cell engraftments into multiple tissues of adult healthy mice, achieved without suppressing the animals’ immune systems. Fetal cells from the livers of male C57Bl/6J Black lineage mice at day 13 to 15 of gestation were injected intravenously into female adult CC57W/MY White mice. The grafting was evaluated by Y-chromosome-specific PCR, cytometric analysis of fluorescently stained donor cells, and histological analysis. All the methods consistently showed the presence of multiple engraftments randomly distributed through the various organs of the recipients. After 60 days, the grafts still constituted 0.1 to 2.75% of the tissues. The grafted cells did not change their appearance in any of the organs except the brain, where they became enlarged. Inflammatory reactions were not detected in any of the histological preparations. The frequency of engraftments was higher in the liver, indicating that similarity between the donor and recipient cells facilitates engraftment. The high inherent plasticity of fetal liver cells underlies their ability to integrate into healthy recipient organs, which can be governed by environmental conditions and connections with neighboring cells rather than by the initial cellular developmental programs. The fact that fetal liver cells can be grafted into multiple tissues of healthy animals indicates that they can be used to replace the natural loss of cells in adult organisms.
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