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Reproduction of the European hare in Patagonia, Argentina

100%

Bonino N., Montenegro A.

Acta Theriologica

1997

tom 42

nr 1

Monthly samples totalling 202 European hares Lepus europaeus Pallas, 1778 were collected in southern Argentina from August 1993 to March 1994. The sex ratio of the whole sample was 1:1.2 in favour of females. Pregnant females were present from August to February and lactating females were present from September to March. The percentage of pregnant females was 59% for the whole breeding season. The mean ovulation rate for female hares was 2.78; the number of ova ovulated was not significantly correlated with body weight. The mean number of implantation sites per reproducing female was 2.46. The number of embryos in a litter varied from 1 to 4, the average being 2.12. The prenatal mortality was 56.5% of all ova ovulated and of these 34.7% were lost before implantation and 21.8% after implantation.

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Plant regeneration from immature inflorescence-derived callus of Italian ryegrass [Lolium multiflorum Lam.]: chromosome number and fertility of regenerated plants

80%

Zwierzykowski Z., Rybczynski J. J.

Journal of Applied Genetics

1998

tom 39

nr 3

Twenty field-grown genotypes of diploid Italian ryegrass (Lolium multiflorum Lam., 2n = 2x = 14) were tested for their ability to induce callus and regenerate plants. Callus cultures were initiated from segments of immature inflorescences cultured on the MS medium supplemented with 4.0 mg L⁻¹ 2,4-D. The calluses were subcultured first on the maintaining medium (MS medium with 2.0 mg L⁻¹ 2,4-D) and later on the rooting medium (MS medium with 0.2 mg L⁻¹ 2,4-D). The frequency of callus induction varied depending on the source of explant and the initial genotype. A total of 473 green plantlets were regenerated, of which 420 were established in the soil. All these plants had the morphological characteristics of Italian ryegrass. Among 372 regenerants analysed cytologically, 302 (81.2%) had the expected diploid chromosome number (2n = 2x= 14), 65 (17.5%) were tetraploid (2n = 4x = 28); several aneuploids and mixoploids were also observed. All diploid and tetraploid regenerants were male and female fertile. However, a great variation of female fertility within and between both groups of regenerants was observed.

Ograniczanie wyników

1 Acta Theriologica

1 Journal of Applied Genetics

1 Bonino N.

1 Montenegro A.

1 Rybczynski J. J.

1 Zwierzykowski Z.

1 1998

1 1997

Wyniki wyszukiwania

Reproduction of the European hare in Patagonia, Argentina

Plant regeneration from immature inflorescence-derived callus of Italian ryegrass [Lolium multiflorum Lam.]: chromosome number and fertility of regenerated plants