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The release of sludge-born bacteria and their further subsurface transport was studied. The migration of bacteria was investigated in column experiments which were carried out under the conditions correspond­ing to naturally occurring extreme rainfall. Coal fly ash as well as coarse and medium grained sand, whose properties are similar to the soils present in degraded areas, were used as column beds. Sewage sludge was applied on the top of column beds in the quantity corresponding to the best land-reclamation practice. Clos­tridium perfringens and fecal coliforms were used as bio-tracers of fecal pollution. The obtained results showed the dynamic of bacterial cells' leaching from the sludge matrix, and the dynamic of their infiltration through the column beds to the effluents. The bacterial breakthrough curves obtained for the fly ash and for the sandy media differ significantly, reflecting the differences in transport processes and in the survival of bacterial cells. It has been found that the fly ash layer, whose thickness equals 0.80 m, can be regarded as an effective filter, which limits bacterial migration. When sludge is applied to the sandy soils, about 0.02% of the initial number of sludge bacteria can migrate downwards the sandy layer and can cause contamination of potentially shallow aquifers.
A total of 138 E. coli isolates obtained from fecal samples of various mammals and birds and water samples of different origin were compared. The comparison included multiple antibiotic resistance to a total of 21 antibiotics, the protein profile (PAGE) of the isolates, the DNA fragments of each isolate after treatment with the restriction enzymes, HindIII, EcoRI, EcoRV, BamHI and BglII and the presence of plasmid(s). Our results indicated that human, horse and Nile water isolates were resistant to 62% of the antibiotics used, while cat isolates were resistant to 47%, mouse isolates 72%, avian to 34%, Ismailia canal 67%, household water reservoir 81% and swimming pool isolates were resistant to 57%. Protein profile (PAGE) analysis is the second method used; the protein with 20.29 kd molecular weight is common in avian, household water reservoir and swimming pool isolates. The third method includes DNA fragments using different restriction enzymes HindIII, EcoRI, EcoRV, BamHI and BglII.
This paper presents the results of microbiological research on the water of the River Vistula between Wyszogród and Toruń, as regards the total number of planktonic bacteria (TNB), the number of heterotrophic bacteria: psychrophilic (TVC 20oC) and mesophilic (TVC 37oC), the occurrence of anaerobic spore-forming bacteria, and the number of coliform bacteria, faecal coliforms and faecal streptococci. The value of the Korsh coefficient and the titre of coli indicate that the waters of the Lower Vistula on the Wyszogród – Toruń section are moderately polluted and in the majority of cases may be classified as class III purity, with the exception of sections of the river near Płock and Toruń, which are unclassified. The values of the FC/FS ratio indicate the predominance of faecal contamination of human origin.
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