The aim of this study was to assess the course of some parameters of non-specific immunity during the estrus cycle in bitches. Blood samples were collected from 28 healthy bitches at different stages (anestrus, proestrus, estrus, metestrus) of the estrus cycle. Nitroblue tetrazolium (NBT) reduction by blood neutrophils, phagocytic activity of neutrophils, proliferative response of blood lymphocytes to phytohemagglutynin, serum activity of lysozyme and ceruloplasmin, level of total protein and γ-globulin fraction were determined. The study showed that nitroblue tetrazolium (NBT) reduction by blood neutrophils, proliferative response of blood lymphocytes to phytohemagglutynin, serum activity of lysozyme and ceruloplasmin were significantly higher (p ≤ 0.01) in proestrus and estrus than in anestrus.
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