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1

Milk as a new diagnostic tool for rapid detection of fascioliasis in dairy goats using excretory/secretory antigen

100%
Saad M. F., Attia M. M.
Acta Parasitologica
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2021
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tom 66
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nr 2
2

Mass spectrometry analysis of the excretory-secretory (E-S) products of the model cestode Hymenolepis diminuta reveals their immunogenic properties and the presence of new E-S proteins in cestodes

84%
Bien J., Salamatin R., Sulima A., Savijoki K., Conn D. B., Nareaho A., Mlocicki D.
Acta Parasitologica
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2016
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tom 61
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nr 2
3

A survey on the adjuvant role of naloxone alone or combined with alum in vaccination against fasciolosis in BALB/c mice

84%
Azizi H., Mirzaeei H., Bagheri A., Bazi A., Khamesipour A., Yaghoobi H., Mirzapour A., Khatami M., Elikaee S.
Acta Parasitologica
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2019
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tom 64
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nr 2
4

Oral inoculation of live or dead third-stage larvae of Anisakis simplex in rats suggests that only live larvae induce production of antibody specific to A. simplex

84%
Abe N., Teramoto I.
Acta Parasitologica
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2014
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tom 59
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nr 1
Live Anisakis simplex third-stage larvae (L3) penetrate gastrointestinal mucosa after they are ingested in raw or undercooked seafood, thereafter causing gastrointestinal manifestations and allergic manifestations such as urticaria and anaphylaxis. These allergic reactions are mediated by specific IgE to L3 allergens, especially excretory-secretory (ES) allergens. Recent evidences suggest that only live larvae can cause allergic reactions, although cases attributable to ingestion of cooked, frozen seafood have been reported. Therefore the risk of Anisakis-associated hypersensitivity by ingestion of properly cooked and frozen fish remains controversial. No prior report describes the kinetics of antibody production in experimental animals after oral inoculation with dead L3. This study used ELISA to assess antibody production in rats inoculated orally with dead L3. Positive absorbance value in IgG, IgM, and IgE specific to ES antigen from L3 were found in rats inoculated with live L3 but not with dead L3 (frozen, heated, cut, or homogenized). At one week post re-inoculation with live or frozen L3 to the initially sensitized rats, the absorbance value of the specific IgM and IgE to ES antigen elevated quickly and highly in rats that had been re-inoculated with live L3, but they decreased slightly or did not change in rats inoculated with frozen L3. These results suggest that only ingestion of live L3 can produce the specific antibody and induce initial and secondary sensitizations to L3.
5

Humoral response of mice infected with Toxocara canis following different infection schemes

84%
Novak J., Panska L., Machacek T., Kolarova L., Horak P.
Acta Parasitologica
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2017
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tom 62
|
nr 4
6

Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niteroi, Brazil

84%
Junior I. F., Vericimo M. A., Cardoso L. R., Clemente S. C. S., Nascimento E. R., Teixeira G. A. P. B.
Acta Parasitologica
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2013
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tom 58
|
nr 3
7
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The estimation of different ELISA procedures for serodiagnosis of human trichinellosis

67%
Moskwa B., Bien J., Cabaj W., Korinkova K., Koudela B., Kacprzak E., Stefaniak J.
Wiadomości Parazytologiczne
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2006
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tom 52
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nr 3
Introduction. The most important confirmative diagnostic test for trichinellosis is the presence of the muscle larvae in a tissue biopsy but this direct method has a low sensitivity of light and moderate infections. The aim of presented study was to compare the usefulness of the results obtained by three ELISA procedures for Trichinella spp. diagnosis in human outbreaks. Materials and methods. All sera (cases and controls) were tested for anti-Trichinella antibodies (immunoglobulin G) using commercially available Novatec KIT and two other ELISA procedures based on excretory-secretory (ES) antigens on Trichinella spiralis muscle larvae. The main differences in ELISA procedures were: the protein concentration in antigen, dilution of human serum samples, conjugate and the time of conjugate incubation. Additional differences were noticed in ES antigen preparation procedures as well as in T. spiralis isolates used in these procedures. Serum samples were obtained from 22 symptomatical patients from Poznań region (West Poland), geographic area where human outbreak had occurred. Control serum samples were obtained from 20 patients from an open population from a non endemic trichinellosis area. Results. The results were analyzed in terms of both: statistical and epidemiological point of view. Linear regression analysis and correlations coefficient r between OD values of total 22 patients obtained in three ELISA procedures were positive and high statistically significant. Three ELISA procedures revealed different cut-off values and positivity rates for outbreak. However, the majority of positive samples were found as positive in three procedures, but some of them were positive in two or one procedure only. These individual variability in sera reactivity observed in three ELISA procedures could be very important from epidemiological point of view.
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