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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In situ genetic variability and micropropagation of Cerastium banaticum (Rochel) Heuff. (Caryophyllaceae)– a rare and endemic species from Romania

100%
Cristea V., Besenyei E., Jarda L., Farkas A., Marcu D., Clapa D., Halmagyi A., Butiuc-Keul A.
Acta Biologica Cracoviensia. Series Botanica
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2019
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tom 61
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nr 1
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions

80%
Marcinek B., Parzymies M., Poniewozik M., Kozak D., Durlak W.
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
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nr 5
The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.
3
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An optimized micropropagation protocol by ex vitro rooting of Pear rootstock OHF 333 (Pyrus communis L.)

80%
Dimitrova N., Nacheva L.
Acta Agrobotanica
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2021
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tom 74
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nr 1
4
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In vitro proliferation and ex vitro rooting of microshoots of lisianthus using auxin and cytokinin on solid, liquid and double-phase culture systems

70%
Kaviani B., Bahari B.
Acta Scientiarum Polonorum. Hortorum Cultus
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2019
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tom 18
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nr 4
A protocol was developed for high frequency and low cost of in vitro shoot proliferation and ex vitro rooting of Eustoma grandiflorum (Gentianaceae) on solid medium. Shoot tips as explants were cultured on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.00, 0.01, 0.10 and 1.00 mg l–1) and 6-benzylaminopurine (BAP) (0.00, 0.50, 2.00 and 5.00 mg l–1). Three culture media systems (solid, liquid and double-phase) were applied. None of the explants cultured on liquid and double-phase media resulted in live plant production. Maximum axillary shoot number (54.45) was recorded in the plantlets treated with 0.10 mg l–1 2,4-D in combination with 5.00 mg l–1 BAP. Treatment of 0.01 mg l–1 2,4-D along with 0.50 mg l–1 BAP produced maximum node number and internode length. Some shoots produced on medium containing plant growth regulators (PGRs) were rooted in soil. The largest number (5.50/plantlet) and longest length of root (7.75 cm/plantlet) were obtained in ex vitro condition on the base of shoots produced in culture medium enriched with 0.10 mg l–1 2,4-D along with 0.50 mg l–1 BAP. The combination of 1.00 mg l–1 2,4-D and 0.50 mg l–1 BAP was found to be the most suitable PGRs for obtaining the highest callus weight. The most fresh weight was calculated from plantlets grown on the medium containing 0.10 mg l–1 2,4-D along with 5.00 mg l–1 BAP. Maximum dry weight was obtained in free-PGRs medium. About 90% of the rooted plantlets were established successfully in cultivation beds. Acclimatized plants were morphologically similar to the mother plants.
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