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A rapid method of agarose gel electrophoresis for separation of different conformational forms of DNA

100%

Ciesielska E., Szmigiero L.

Cellular and Molecular Biology Letters

1996

tom 01

nr 4

A fast two step method of agarose gel electrophoresis for separation of different conformational forms of DNA is described. In the first step the gel is run in the buffer without ethidium bromide and then the gel is stained with that dye. After staining the second step of electrophoresis is performed in the buffer without ethidium bromide. This two step procedure allows one to receive very good resolution between bands corresponding to relaxed, supercoiled, open circular, and linear forms of DNA.

Densitometric quantification of DNA topoisomers in ethidium bromide-stained agarose gels and chemiluminescence-detected X ray films

86%

Topcu Z.

Acta Biochimica Polonica

2000

tom 47

nr 3

This study investigates the contribution of deformational strain imposed by topological interconversions of DNA in ethidium bromide-binding on agarose gels. Closed-circular plasmid DNAs were nicked using UV exposure and the DNA bands were quantified by densitometry. The results show that the closed circular DNA binds the same amount of the dye as its nicked counterpart. The relationship between the band intensity on X-ray films of chemiluminescence-detected Southern blots and DNA concentration was shown to be linear.

Respiratory deficient mutants of xylose-fermenting yeast - obtainment and features

58%

Chmielewska J., Dziuba E.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

2003

tom 06

nr 2

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Cellular and Molecular Biology Letters

1 Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

1 Chmielewska J.

1 Ciesielska E.

1 Dziuba E.

1 Szmigiero L.

1 Topcu Z.

1 2003

1 2000

1 1996

Wyniki wyszukiwania

A rapid method of agarose gel electrophoresis for separation of different conformational forms of DNA

Densitometric quantification of DNA topoisomers in ethidium bromide-stained agarose gels and chemiluminescence-detected X ray films

Respiratory deficient mutants of xylose-fermenting yeast - obtainment and features