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Mead is a traditional alcoholic beverage obtained by fermenting mead wort; however, its production still remains frequently an empirical exercise. Different meads can be produced, depending on fermentation conditions. Nevertheless, to date few studies have been developed on factors that may influence mead quality. The main objective of this work was to study the influence of sweetness and ethanol content on mead acceptability. Different meads were produced with two sweetness levels (sweet and dry meads) and three ethanol contents (18, 20, 22% (v/v)), adjusted by brandy addition. Afterwards, meads acceptability was evaluated by sensory analysis through a consumers' panel (n=108) along with chemical analysis by HPLC-RID of glucose, fructose, ethanol, glycerol and acetic acid. The sweet (75 gglncose+[rnctose/L) and dry (23 gglncose+[rnctose/L) meads presented glycerol contents equal to 5.10±0.54 and 5.96±0.95 g/L, respectively, that were desirable since glycerol improves mead quality. Low concentrations of acetic acid were determined (0.46±0.08 and 0.57±0.09 g/L), avoiding the vinegar off-character. Concerning sensory analysis, the alcohol content of mead had no effect on the sensory attributes studied, namely, aroma, sweetness, flavour, alcohol feeling and general appreciation. Regarding sweetness, the "sweet meads" were the most appreciated by the consumers (score of 5.4±2.56), whereas the "dry meads" (score of 2.7±2.23) showed low acceptability. In conclusion, this work revealed that sweetness is a sensory key attribute for mead acceptance by the consumers, whereas ethanol content (18 to 22% (v/v)) is not.
Alcoholic emulsion creams are characterized with high viscosity and density, which makes the alcohol content and other physical and chemical parameters determination of products significantly difficult. Analytical methods applied for the determination of ethanol, are labor-consuming, they are characterized with low precision, require the sample distillation and the achieved results are mostly underestimated. On a base of comparison of various distillation techniques and determination methods, it can be stated that ethanol concentration measurements in alcoholic creams using pycnometric and DMA-58 density-meter after previous sample distillation (100 cm3 of cream + 200 cm3 of water), are distinguished with relatively high precision and result repeatability. Also applying the SPME extraction in gaseous phase along with chromatographic analysis seems to be advantageous. The method based on the refractometric measurement of toluene and benzene extracts of egg emulsions is characterized with short performance time, sufficient precision and very low costs.
This study examines the effects of ethanol on lindane-induced seizures in rats. The animals were divided into following groups: 1. saline, 2. DMSO (dimethylsulfoxide), 3. lindane dissolved in DMSO in the dose of 4, 6 or 8 mg/kg (L4, L6 and L8 groups, respectively), 4. ethanol 2 g/kg administered 30 min prior to lindane (protected groups AL4, AL6 and AL8) and 5. ethanol alone (2 g/kg). In order to determine ethanol concentration in plasma, blood samples were collected by cardiac puncture 30 and 60 min after ethanol injection. For EEG and power spectra recordings, electrodes were implanted into the skull. The lindane treatment resulted in a dose-dependent increase of seizure incidence and severity. The rats displayed severe seizure patterns characterized by high voltage spike-wave complexes, poly-spikes and sleep-like patterns in EEG, while the power spectra were intensively elevated in comparison to the corresponding controls. Ethanol alone led to increased EEG power spectra, which became dominant in the range of 0-4 Hz. For evaluation of anticonvulsant ethanol action we compared latency to seizure, incidence and seizure severity (scale from 0 to 4) in the examined groups. Ethanol diminished seizure incidence in AL4 and AL6 groups, decreased intensity of convulsions, and prolonged duration of latency period in AL8 group. We observed suppression of the EEG signs of lindane-provoked epileptiform activity in AL4 and AL6, but not in AL8 group. These results suggest that ethanol acted protectively on lindane-induced seizures and suppressed behavioral and epileptic EEG spiking activity.
The influence of the addition of yeast cell wall to the high-sugar musts on the process of wine fermentation was investigated. The yeast Saccharomyces bayanus S.o./1 and S.o./1AD as well as the strain Tokay was used. Suplementation on 21 days;, allowed to obtain in wines produced by yeast S.o./1 and S.o./1AD 0,4% v/v ethanol more than in the control wines, but in wines fermented by Tokay strain any influence on the alcohol content was stated. In the case of the yeast Tokay yeast cell wall was added on 7 days of fermentation too. It was found then favourable influence on the alcohol content, process efficiency as well as a number and a viability of yeast cells.
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