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A simple method for the determination of the cholesterol esterase activity

100%

Stepien A. E., Gonchar M.

Acta Biochimica Polonica

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2013

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tom 60

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nr 3

The proposed method determines the activity of cholesterol esterase (CEH) and takes advantage of its ability to catalyze the hydrolysis of cholesterol esters naturally present in human serum. The assay is based on Allain's method of spectrophotometric determination of cholesterol by means of cholesterol oxidase, peroxidase, but using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) as phenolic chromogen and human serum as a source of substrate for the CEH as a novelty. Furthermore, it is characterized by low costs and high precision. It can be employed to control the activity of CE preparations used for the preparation of enzymatic kits for the determination of cholesterol or for screening of potential bacterial enzyme producers.

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The effect of feeding on aminopeptidase and non-specific esterase activity in the digestive system of pike-perch (Sander lucioperca L.)

84%

Kamaszewski M., Ostaszewska T.

Annals of Warsaw University of Life Sciences - SGGW. Animal Science

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2013

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tom 52

The pike-perch (Sander lucioperca L.) at the age of 18 days were fed for 21 days using three different diets: Agio Norse (An), casein-gelatin (Cas), cod meal with gelatin (Mac) and nauplius Artemia salina (Art - control diet). On the last day of the experiment, fish fed Art and An diets had the statistically significant highest body mass, length, and survival. On the last day, the highest aminopeptidase activity in the anterior intestine and posterior intestine was registered in fish fed nauplius Artemia salina. The lowest activity of this enzyme in the anterior intestine was to be found in fish fed with Cas diet, while there was no difference between among groups in the posterior intestine. The non-specific esterase activity was registered in the stomach, liver, anterior intestine as well as in the posterior intestine. The lowest activity of this enzyme in the stomach was observed in the pike-perch fed with the Cas diet. In the anterior intestine, the highest activity was registered in fish fed with Art, and the lowest - on the Cas and Mac diet. The results of the current research prove that feeding the pike-perch An diet has a positive effect of the survival of the pike-perch, their growth rate and the activity of the enzymes. By contrast, feeding pike-perch Cas and Mac diets did not satisfy nutritional needs of fish, resulting in their low survival, growth rate and low activity of the enzymes examined.

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Enzymes of protein breakdown in germinating Triticale grains resistant and susceptible to pre-harvest sprouting

84%

Bielawski W., Dojczew D., Kaczkowski J., Kolbuszewska-Podres W.

Acta Physiologiae Plantarum

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1994

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tom 16

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nr 1

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Acta Physiologiae Plantarum

1 Annals of Warsaw University of Life Sciences - SGGW. Animal Science

1 Bielawski W.

1 Dojczew D.

1 Gonchar M.

1 Kaczkowski J.

1 Kamaszewski M.

1 Kolbuszewska-Podres W.

1 Ostaszewska T.

1 Stepien A. E.

2 2013

1 1994

A simple method for the determination of the cholesterol esterase activity

The effect of feeding on aminopeptidase and non-specific esterase activity in the digestive system of pike-perch (Sander lucioperca L.)

Enzymes of protein breakdown in germinating Triticale grains resistant and susceptible to pre-harvest sprouting