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Expression of genes modulated by epigallocatechin-3-gallate in breast cancer cells

100%

Bogacz A., Wolek M., Juskowiak B., Karasiewicz M., Kaminski A., Uzar I., Polaszewska A., Kostrzewa Z., Czerny B.

Herba Polonica

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2018

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tom 64

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nr 3

Breast cancer is the most common malignant cancer among women. Both drug resistance and metastasis are major problems in the treatment of breast cancer. Therefore, adjuvant therapy may improve patients’ survival and affect their quality of life. It is suggested that epigallocatechin gallate (EGCG) which is well known for its chemopreventive activity and acts on numerous molecular targets may inhibit the growth and metastasis of some cancers. Hence, discovering the metastatic molecular mechanisms for breast cancer may be useful for therapy.The aim of the study was to determine the effect of EGGC on the mRNA expression level of genes such as ZEB1, ABCB1, MDM2, TWIST1 and PTEN in MCF-7 breast cancer cells. MCF7/DOX were cultured in the presence of 0.2 μM DOX and EGCG (20-50 μM). The mRNA expression level was determined by real-time quantitative PCR using RealTime ready Custom Panel 96 kit. Our results showed an important increase (about 2-fold for 20 μM EGCG + 0.2 μM DOX and 2.5-fold for 50 μM EGCG + 0.2 μM DOX, p<0.05) in ZEB1 expression levels. In case of ABCB1 gene lack of influence on the mRNA level was observed (p>0.05). We also observed significant decrease of ZEB1 expression in MCF7 cells with 20 μM and 50 μM EGCG (p<0.05). In addition, EGCG (20 μM) caused an increase of MDM2 and PTEN mRNA levels in almost 100% (p<0.05) and 40% (p>0.05), respectively. Lack of the influence of EGCG was noted for the TWIST1 gene expression. In case of MCF7/DOX we showed an increase of mRNA level of PTEN gene about 50% (p<0.05). These results suggest that EGCG may be potentially used in adjuvant therapy in the breast cancer treatment.

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Induction of apoptosis in vitro using epigallocatechin-3-gallate [EGCG] in selected tumour cells

86%

Borska S., Gebarowska E., Wysocka T., Drag-Zalesinska M., Zabel M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

3

Effects of cigarette smoke borne reactive nitrogen species on salivary alpha-amylase activity and protein modifications

58%

Weiner D., Khankin E. V., Levy Y., Reznick A. Z.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 5

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Induction of apoptosis by EGCG in selected tumour cell lines in vitro

58%

Borska S., Gebarowska E., Wysocka T., Drag-Zalesinska M., Zabel M.

Folia Histochemica et Cytobiologica

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2003

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tom 41

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nr 4

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Folia Histochemica et Cytobiologica

1 Herba Polonica

1 Journal of Physiology and Pharmacology. Supplement

2 Borska S.

2 Drag-Zalesinska M.

2 Gebarowska E.

2 Wysocka T.

2 Zabel M.

1 Bogacz A.

1 Czerny B.

1 Juskowiak B.

1 Kaminski A.

1 Karasiewicz M.

1 Khankin E. V.

1 Kostrzewa Z.

1 Levy Y.

1 Polaszewska A.

1 Reznick A. Z.

1 Uzar I.

1 Weiner D.

1 Wolek M.

1 2018

1 2009

1 2003

1 2002

Expression of genes modulated by epigallocatechin-3-gallate in breast cancer cells

Induction of apoptosis in vitro using epigallocatechin-3-gallate [EGCG] in selected tumour cells

Effects of cigarette smoke borne reactive nitrogen species on salivary alpha-amylase activity and protein modifications

Induction of apoptosis by EGCG in selected tumour cell lines in vitro