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High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

100%

Silva F. M. P., Lopes R. S., Bresciani K. D. S., Amarante A. F. T., Araujo Jr J. P.

Acta Parasitologica

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2014

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tom 59

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nr 1

In this paper we report the occurrence of zoonotic species of Cryptosporidium (C. ubiquitum and C. parvum subtype IIaA15G2R1) and the host-specific Giardia duodenalis genotype E in a sheep farm from Brazil. Fecal specimens were collected from 100 animals and screened for the presence of G. duodenalis and Cryptosporidium spp. by enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequence analysis of selected samples was used to confirm the species and to characterize the isolates. Of 100 specimens, 34 were positive for Giardia (34%) and 25 were positive for Cryptosporidium (25%). The prevalence of Giardia and Cryptosporidium was higher in lambs than in ewes. Co-infection was observed in 14% of animals. All G. duodenalis isolates belonged to genotype E. The genetic characterization performed in 22 Cryptosporidium-positive samples revealed the presence of zoonotic C. ubiquitum and C. parvum in 76% (19/25) and 12% (3/25) of infected animals, respectively. The presence of zoonotic Cryptosporidium subtypes in lambs was of epidemiological and public health relevance.

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Immunoenzymatic method for progesterone detection in bovine urine, muscles and serum by means of a commercial EIA test used in human medicine

100%

Wozniak B., Wojton B.

Bulletin of the Veterinary Institute in Puławy

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1995

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tom 39

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nr 2

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Possible involvement of cAMP and protein phosphorylation in the cell signaling pathway for resting cyst formation of ciliated protozoan Colpoda cucullus

80%

Sogame Y., Asami H., Kinoshita E., Matsuoka T.

Acta Protozoologica

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2011

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tom 50

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nr 2

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Zastosowanie folii PVC produkcji krajowej, jako nosnika w tescie ultramikro Elisa do wykrywania wirusow ziemniaka M i Y. Komunikat

80%

Mierzwa Z., Zurek H., Marciniak-Dziopa M.

Biuletyn Instytutu Ziemniaka

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1993

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nr 42

23-27

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Hyperhomocysteinemia among Omani autistic children: a case-control study

80%

Ali A., Waly M. I., Al-Farsi Y. M., Essa M. M., Al-Sharbati M. M., Deth R. C.

Acta Biochimica Polonica

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2011

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tom 58

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nr 4

High serum homocysteine (Hcy) level is regarded as an indicator for impairment of folate-dependent methionine cycle and is associated with oxidative stress. In a case control study, we evaluated eighty 3-5 years old Omani children (40 diagnosed with Autism Spectrum Disorder and 40 their age and gender matched controls) for their fasting serum homocysteine levels as a biomarker of Autism Spectrum Disorder (ASD). Serum folate and vitamin B12 status were also evaluated. The serum homocysteine was measured using an enzyme immunoassay (EIA) technique whereas folate and vitamin B12 were measured using an automated random access immune-assay system. The results indicated that mean serum Hcy levels were significantly (P < 0.05) higher in autistic children (20.1 ± 3.3 µmol/L) as compared to controls (9.64 ± 2.1 µmol/L). Significantly (P < 0.05) lower serum folate (1.8 ± 0.4 µg/L) and vitamin B12 (191.1 ± 0.9 pg/mL) levels were observed in autistic children as compared to controls (6.1 ± 0.6 µg/L and 288.9 ± 1.3 pg/mL, respectively). The levels of homocysteine in autistic children were also much higher as compared to normal reference values (5-15 µmol/L). The results suggest that high fasting serum homocysteine and low folate and vitamin B12 levels could be used as clinical biomarkers for an early diagnosis and management of ASD.

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Quantitative determination of antyamylolitic and antyproteolitic activities as well as gluten content in new lines of cereal seeds

80%

Warchalewski J. R., Kusnierz R., Piasecka-Kwiatkowska D., Klockiewicz-Kaminska E., Madaj D., Gralik J.

Food Science and Technology. Scientific Papers of Agricultural University of Poznan

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1998

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