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Test Elisa do wykrywania enterotoksyny LT szczepow Escherichia coli

100%

Osek J.

Medycyna Weterynaryjna

1996

tom 52

nr 11

705-707

The aim of the study was to develop a sensitive and specific ELISA for the detection of LT enterotoxin in E. coli strains. The test was based on the antitoxic antibodies obtained from rabbits immunized with В subunit of cholera toxin (CT). The toxin was structurally closely related with LTB of E. coli. CTB was expressed in a V. cholerae strain harboring a plasmid with a ctxB gene. The toxin was isolated and purified from a growth medium by means of precipitation. A goat antirabbit IgG labeled with HRP antibodies was used as a conjugate. The developed test turned out to be highly specific and sensitive: it was possible to detect from 1.5 to 4.0 ng of LTB. One hundred strains of E. coli isolated from piglets were tested by the LT ELISA. It was found that 67 strains (85.9%) isolated from piglets with diarrhea were LT+ whereas only 2 strains (9.1%) from healthy piglets proved to be enterotoxigenic.

Evaluation of pathogenicity determinants of Yersinia pseudotuberculosis strains isolated from chinchillas from the Western Pomerania area

100%

Emirsajlowicz-Zalewska W., Furowicz A. J., Aleksic S., Czernomysy-Furowicz D.

Advances in Agricultural Sciences

1996

tom 05

nr 1

19-24

Escherichia coli - bakteria o wielu "twarzach"

70%

Wrobel P.

Trzoda Chlewna

2015

tom 53

nr 12

Ograniczanie wyników

1 Advances in Agricultural Sciences

1 Medycyna Weterynaryjna

1 Trzoda Chlewna

1 Aleksic S.

1 Czernomysy-Furowicz D.

1 Emirsajlowicz-Zalewska W.

1 Furowicz A. J.

1 Osek J.

1 Wrobel P.

1 2015

2 1996

Wyniki wyszukiwania

Test Elisa do wykrywania enterotoksyny LT szczepow Escherichia coli

Evaluation of pathogenicity determinants of Yersinia pseudotuberculosis strains isolated from chinchillas from the Western Pomerania area

Escherichia coli - bakteria o wielu "twarzach"