The present study has examined the level of total IgA and antigen-specific IgA antibodies in serum and bile in various inbred strains of mice infected with Trichinella spiralis. These strains of mice differ in the speed at which they expel the adult worms from the gut. BALB/c and CBA mice expel adult worms faster than C3H and C57BL/6 mice. However, the CBA strain of mice is more resistant to the establishment of an initial infection of T. spiralis than BALB/c mice. While total serum IgA and bile sIgA concentrations correlated with the time course of the expulsion of adult worms, there was no similar correlation between IgA concentrations and the intensity of T. spiralis infection during the muscle phases of infection in any of the strains of mice investigated. Specific IgA antibodies in sera and sIgA antibodies in bile were measured by ELISA in C57BL/6 mice using crude somatic L1 muscle larvae (AgL1) and crude adult worm (AgAd) antigens. A pronounced increase in sIgA antibodies to AgL1 antigen was found by day 9 of infection in bile. However, a gradual increase in IgA in serum to AgAd antigen was observed from 6 till 24 DAI. Specific IgA response in serum to AgAd was much higher than to AgL1 and, in contrast the sIgA response in bile, was more pronounced to AgL1 than to AgAd. This result suggests that bile may also provide a valuable source of sIgA.
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.