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Mapowanie bialek plazmy nasienia knura przy wykorzystaniu metody elektroforezy dwukierunkowej w zelu poliakryloamidowym

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Kordan W., Strzezek J., Soliwoda D., Lecewicz M., Mogielnicka M.

Medycyna Weterynaryjna

2008

tom 64

nr 02

223-226

An attempt was made to use a modified 2-D PAGE technique to analyze seminal plasma proteins and their polymorphisms in relation to boar age and season. The 2-D PAGE analysis of seminal plasma proteins was performed using a buffer pH gradient of 3 to 10. Modifications to the 2-D PAGE procedure included substituting mercaptoethanol (ME) with dithiothreitol (DTT) and the use of a specific reagent assay (Plus One 2-D Clean-Up Kit, Amersham Biosciences), which markedly improved the resolution of the electrophoregrams. Polymorphisms by polypeptide mapping of boar seminal plasma were dependent on the animal age and season. Furthermore, the amount of polypeptides detected in the seminal plasma was significantly lower in 12 month-old boars compared with 3 year-olds. Additionally, the seminal plasma polypeptides were markedly lower in the summer than in the autumn. The results of the study showed that mapping seminal plasma proteins may be used as a marker for the secretor activity of boar accessory sex glands, and as a selection criterion for male reproduction.

Ograniczanie wyników

1 Medycyna Weterynaryjna

1 Kordan W.

1 Lecewicz M.

1 Mogielnicka M.

1 Soliwoda D.

1 Strzezek J.

1 2008

Wyniki wyszukiwania

Mapowanie bialek plazmy nasienia knura przy wykorzystaniu metody elektroforezy dwukierunkowej w zelu poliakryloamidowym