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The objective of this study was to examine the association between β-hydroxybutyrate (BHBA) and glucose, calcium (Ca), phosphorus (P), and vitamin C concentrations in dairy healthy and with subclinical ketosis cows during an early lactation period. The blood from 99 healthy cows and 26 sub-clinically ketotic cows within the first two months of lactation was sampled. Serum concentration of BHBA was measured by enzymatic-rate method, glucose by oxidase method, and P by colorimetric method. These tests were performed in a biochemical auto-analyser. Serum Ca concentration was measured by atomic absorption spectrometer followed by acid digestion. Vitamin C was measured by HPLC. The results showed that in ketotic cows serum BHBA was significantly increased (P<0.01) and glucose and Ca concentrations were significantly decreased (P<0.01) as compared to healthy cows, while vitamin C and P concentrations were similar in the both groups of cows (P>0.05). The serum BHBA concentration was negatively correlated with the serum glucose and Ca concentrations (P<0.01). Vitamin C and P status of cows with subclinical ketosis was not affected by BHBA. Serum Ca concentration can be used as a reference index for the diagnosis of subclinical ketosis.
The aim of this paper was to investigate the impact of clinical lameness in Polish Holstein-Friesian dairy cows on milk yield as well as on fat, protein, and lactose yields during early lactation (120 d). The dataset includes 312 monthly test-day milk yields and milk composition records from 78 cows, which calved from October 2008 to April 2009. Twice a week, during the first 120 d postpartum, the cows were examined for lameness and they were scored fortnightly according to a 5-point locomotion scoring system. Factors affecting milk yield and milk components included calving season, parity, month of lactation, and degree of lameness. In cows, which were clinically lame for a month and which were clinically lame for more than a month (maximum per 2 months), the total mean reduction in milk yield per 120-d lactation was approximately 308 and 283 kg, respectively, as compared with cows, which were never lame in early lactation.
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